Protein Info for HER17_RS13465 in Pectobacterium carotovorum WPP14

Annotation: adenosylmethionine--8-amino-7-oxononanoate transaminase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 434 TIGR00508: adenosylmethionine-8-amino-7-oxononanoate transaminase" amino acids 13 to 427 (415 residues), 618.6 bits, see alignment E=2.2e-190 PF00202: Aminotran_3" amino acids 27 to 426 (400 residues), 419.8 bits, see alignment E=4.8e-130

Best Hits

Swiss-Prot: 74% identical to BIOA_SERMA: Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (bioA) from Serratia marcescens

KEGG orthology group: K00833, adenosylmethionine-8-amino-7-oxononanoate aminotransferase [EC: 2.6.1.62] (inferred from 94% identity to eca:ECA2826)

Predicted SEED Role

"Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62)" in subsystem Biotin biosynthesis (EC 2.6.1.62)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.62

Use Curated BLAST to search for 2.6.1.62

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (434 amino acids)

>HER17_RS13465 adenosylmethionine--8-amino-7-oxononanoate transaminase (Pectobacterium carotovorum WPP14)
MSPEDIAFDQRHIWHPYTSMTVPLPCYPVIAASGVELQLDDGRRLIDGMSSWWAAIHGYN
HPAINQAVQAQLSQMSHVMFGGITHPAAVALCRQLVDITPDTLECVFLADSGSVAVEVAM
KMALQYWQARGEARQRFLTLRHGYHGDTFGAMSVCDPQNSMHSLYQGYLPAHLFVDAPPC
AAINEGGFTDDWQEADIAPLQAMLSAHAHEIAAVILEPIVQGAGGMRFYHPAYLRRVREL
CDQWGILLIADEIATGFGRTGKLFACEHAGISPDIMCLGKALTGGYMTLSATLTTRIVAE
TISNGAAGCFMHGPTFMGNPLACAAANASLSLLAENRWQQQVQNIERQLRDALQPCADSP
LVADVRVLGAIGVVETQRPVNMARLQRFFVERGVWIRPFGRLIYLMPPFIIQPDQLRKLT
DAVCAAIRDEQLFD