Protein Info for HER17_RS12300 in Pectobacterium carotovorum WPP14

Annotation: formate transporter FocA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 286 transmembrane" amino acids 35 to 55 (21 residues), see Phobius details amino acids 69 to 98 (30 residues), see Phobius details amino acids 111 to 135 (25 residues), see Phobius details amino acids 161 to 181 (21 residues), see Phobius details amino acids 193 to 216 (24 residues), see Phobius details amino acids 255 to 278 (24 residues), see Phobius details TIGR04060: formate transporter FocA" amino acids 14 to 279 (266 residues), 415.6 bits, see alignment E=7.4e-129 PF01226: Form_Nir_trans" amino acids 15 to 276 (262 residues), 257.9 bits, see alignment E=4.1e-81 TIGR00790: formate/nitrite transporter" amino acids 26 to 280 (255 residues), 309.6 bits, see alignment E=1.5e-96

Best Hits

Swiss-Prot: 81% identical to FOCA_ECOLI: Probable formate transporter 1 (focA) from Escherichia coli (strain K12)

KEGG orthology group: K06212, formate transporter (inferred from 99% identity to pwa:Pecwa_1998)

MetaCyc: 81% identical to formate channel FocA (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-1; TRANS-RXN-381

Predicted SEED Role

"Formate efflux transporter (TC 2.A.44 family)" in subsystem Fermentations: Mixed acid

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (286 amino acids)

>HER17_RS12300 formate transporter FocA (Pectobacterium carotovorum WPP14)
MKADNPFTLLLPAAMAKVAEDAGVYKATKQPCTTFYLAITAGVFISIAFVFYITATTGTA
TIPFGIAKLIGGICFSLGLMLVVVCGADLFTSTVLIVIAKASGRITWKQLACNWANVYVG
NLIGALFFVGLIWFSGEHMVANGAWGLNVLQTAEHKLEHTFVEALCLGILANLLVCLAVW
MSYSGRTLTDKMFAMILPVAMFVSSGFEHSIANMFMIPMGIVIKNFAAPEFWNAIGMVPS
QFEHLTVSHFITDNLIPVTLGNIIGGGVMVGLTYWVIYLRGGDKHH