Protein Info for HER17_RS12295 in Pectobacterium carotovorum WPP14

Annotation: 30S ribosomal protein S12 methylthiotransferase accessory protein YcaO

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 587 TIGR00702: YcaO-type kinase domain" amino acids 11 to 404 (394 residues), 541.6 bits, see alignment E=5.2e-167 PF02624: YcaO" amino acids 59 to 391 (333 residues), 248.6 bits, see alignment E=1e-77 PF18381: YcaO_C" amino acids 407 to 582 (176 residues), 222.8 bits, see alignment E=2.8e-70

Best Hits

Swiss-Prot: 82% identical to YCAO_ECOLI: Ribosomal protein S12 methylthiotransferase accessory factor YcaO (ycaO) from Escherichia coli (strain K12)

KEGG orthology group: K09136, ribosomal protein S12 methylthiotransferase (inferred from 98% identity to pwa:Pecwa_1999)

MetaCyc: 82% identical to ribosomal protein S12 methylthiotransferase accessory factor YcaO (Escherichia coli K-12 substr. MG1655)
ATP diphosphatase. [EC: 3.6.1.8]

Predicted SEED Role

"FIG00634513: hypothetical protein"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.8

Use Curated BLAST to search for 3.6.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (587 amino acids)

>HER17_RS12295 30S ribosomal protein S12 methylthiotransferase accessory protein YcaO (Pectobacterium carotovorum WPP14)
MTQTFIPGKDAALEDSIARFQQQLQDLGFNIEEASWLNPVPNVWSVHIRDRDCPLCFTNG
KGASKKAALASALGEYFERLSTNYFFADFYLGKSIANGDFVHYPNEKWFPIPEDDALPEG
ILDARLHQFYDPEQELSASDLIDLQSGNADRGICALPFTRQSDQQTVYIPMNIIGNLYVS
NGMSAGNTANEARVQGLSEVFERSIKNRIIAESISLPEIPQEVLNRYPGVVEAIATLEKE
GFPIFSYDASLGGKYPVICVVLFNPANGTCFASFGAHPDFGVALERTVTELLQGRGLKDL
DVFTPPTFDDEEVAEHANLETHFIDSSGLISWDMFKKQADYEFADWSFKGSTEEEFATLM
AIFKQEDKEVYIADYEHLAVYACRIIVPGMSDIYPADDLLLANNSMGAHLRDTLLALPDS
KWEKEDYLTLLQQLDDEGLDDFTRVRELLGIATGKDNGWFTLRVGELKAMLALAGGDLDQ
ALSWTEWTQDFNDSVFTAQRSNYYRCLQTLLLLAQEPERDPAEYYDAFTKMYGSETVDAA
SAAVAGETCFYGLFAVDDTFTSLPAHQSLLAAYEKLQQAKRRYWQAK