Protein Info for HER17_RS12260 in Pectobacterium carotovorum WPP14

Annotation: bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01502: PRA-CH
  • amino acids 16 to 88 (73 residues), 110.5 bits, E=2.9e-36
• PF01503: PRA-PH
  • amino acids 98 to 185 (88 residues), 71.3 bits, E=7.1e-24
• TIGR03188: phosphoribosyl-ATP diphosphatase
  • amino acids 98 to 181 (84 residues), 100.9 bits, E=1.7e-33

Best Hits

Swiss-Prot: 83% identical to HIS2_YERPE: Histidine biosynthesis bifunctional protein HisIE (hisI) from Yersinia pestis

KEGG orthology group: K11755, phosphoribosyl-ATP pyrophosphohydrolase / phosphoribosyl-AMP cyclohydrolase [EC: 3.5.4.19 3.6.1.31] (inferred from 98% identity to pct:PC1_1738)

MetaCyc: 81% identical to putative bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase (Escherichia coli K-12 substr. MG1655)
Phosphoribosyl-ATP diphosphatase. [EC: 3.6.1.31]; Phosphoribosyl-AMP cyclohydrolase. [EC: 3.6.1.31, 3.5.4.19]

Predicted SEED Role

"Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19) / Phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31)" in subsystem Histidine Biosynthesis (EC 3.5.4.19, EC 3.6.1.31)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
• L-histidine biosynthesis (10/10 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones
• Histidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.4.19 or 3.6.1.31

Protein Sequence (187 amino acids)

>HER17_RS12260 bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE (Pectobacterium carotovorum WPP14)
MLPVVVQHAVSGEVLMLGYMNQDALQATEESGKVTFFSRTKQRLWTKGESSGHFLNVVSI
TPDCDNDTLLILVNPIGPTCHLGTSSCFSPAASDWTFLYQLEQLLAERKHADPDSSYTAR
LYASGTKRIAQKVGEEGLEAALAATVHDREELTNEAADLMYHLLVLLQDQDLDLATIINR
LKARHNK