Protein Info for HER17_RS12240 in Pectobacterium carotovorum WPP14

Annotation: bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 355 TIGR01261: histidinol-phosphatase" amino acids 3 to 162 (160 residues), 253.2 bits, see alignment E=1.1e-79 TIGR01662: HAD hydrolase, family IIIA" amino acids 4 to 150 (147 residues), 86.4 bits, see alignment E=3e-28 TIGR01656: histidinol-phosphate phosphatase domain" amino acids 5 to 144 (140 residues), 153.9 bits, see alignment E=4.2e-49 PF08645: PNK3P" amino acids 7 to 117 (111 residues), 24.4 bits, see alignment E=4.3e-09 PF13242: Hydrolase_like" amino acids 103 to 152 (50 residues), 33.7 bits, see alignment 5.6e-12 PF00475: IGPD" amino acids 196 to 337 (142 residues), 210.3 bits, see alignment E=2.6e-66

Best Hits

Swiss-Prot: 97% identical to HIS7_PECAS: Histidine biosynthesis bifunctional protein HisB (hisB) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K01089, imidazoleglycerol-phosphate dehydratase / histidinol-phosphatase [EC: 3.1.3.15 4.2.1.19] (inferred from 97% identity to eca:ECA2585)

MetaCyc: 83% identical to imidazoleglycerol-phosphate dehydratase / histidinol-phosphatase (Escherichia coli K-12 substr. MG1655)
Imidazoleglycerol-phosphate dehydratase. [EC: 4.2.1.19]; Histidinol-phosphatase. [EC: 4.2.1.19, 3.1.3.15]

Predicted SEED Role

"Histidinol-phosphatase (EC 3.1.3.15) / Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19)" in subsystem Histidine Biosynthesis (EC 3.1.3.15, EC 4.2.1.19)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.15

Use Curated BLAST to search for 3.1.3.15 or 4.2.1.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (355 amino acids)

>HER17_RS12240 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB (Pectobacterium carotovorum WPP14)
MGQKYLFIDRDGTLIAEPPEDFQVDRLDKLALEPDVIPSLLALQKAGYTLVMITNQDGLG
TESFPQETFDPPHNLMMQILTSQGIRFEQVLICPHLPTDNCTCRKPKTELVTAYLNNGLM
DVANSYVIGDRQTDIQLAQNMGITGLLYQRGGLNWQAITNQLTKRDRHAHVNRVTRETAI
DVNVWLDQEGGSKINTGVGFFDHMLDQIATHGGFRMNIEVKGDLYIDDHHTVEDTGLALG
EALNKALGDKRGIGRFGFVLPMDECLARCALDISGRPHLEYKAEFSYQRVGDLSTEMVEH
FFRSLSYSMACTLHLKTKGRNDHHRVESLFKVFGRTLRQAIRVEGDTLPSSKGVL