Protein Info for HER17_RS12180 in Pectobacterium carotovorum WPP14

Annotation: [citrate (pro-3S)-lyase] ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 354 TIGR00124: [citrate (pro-3S)-lyase] ligase" amino acids 20 to 349 (330 residues), 387.3 bits, see alignment E=5.4e-120 PF00583: Acetyltransf_1" amino acids 36 to 113 (78 residues), 28.4 bits, see alignment E=2.6e-10 TIGR00125: cytidyltransferase-like domain" amino acids 157 to 213 (57 residues), 39.5 bits, see alignment E=4.9e-14 PF08218: Citrate_ly_lig" amino acids 157 to 345 (189 residues), 257.7 bits, see alignment E=8.4e-81

Best Hits

Swiss-Prot: 46% identical to CITC_ECOLI: [Citrate [pro-3S]-lyase] ligase (citC) from Escherichia coli (strain K12)

KEGG orthology group: K01910, [citrate (pro-3S)-lyase] ligase [EC: 6.2.1.22] (inferred from 96% identity to pct:PC1_1752)

MetaCyc: 43% identical to [citrate [pro-3S]-lyase] ligase (Klebsiella pneumoniae)
[Citrate (pro-3S)-lyase] ligase. [EC: 6.2.1.22]

Predicted SEED Role

"[Citrate [pro-3S]-lyase] ligase (EC 6.2.1.22)" (EC 6.2.1.22)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.2.1.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (354 amino acids)

>HER17_RS12180 [citrate (pro-3S)-lyase] ligase (Pectobacterium carotovorum WPP14)
MYANDSVFFDTLDVRLREQERDAVRQFLAHCQLGMDDDIDIVVVGKRGGRLIACAGLAAN
TIKCVAVDPEFRHLNLGVQIVNEVMQQAAQRGHFHLFLYTRPENVDIFRGCGFYPLACYQ
DSAVLMENTPIGIQQYCQSLSAFAHPESLATQADKKIGAIVMNANPFTLGHRYLAEHAAQ
SCDWLHVFVVREDVSFFPFSERLEMVQRGVEHIRNVTVHAGSHYMISKATFPGYFLKEEK
LITRAHAALDLIIFRKYIAPALGITQRFVGTEPFCPVTYQYNQDMHYWLEKDQTVSSPAL
SVVEIERKRQTSGLAISASEVRKLLKLRQYSRIQDIVPASTFAHLQHYYEPEYA