Protein Info for HER17_RS11975 in Pectobacterium carotovorum WPP14

Annotation: intermembrane transport protein PqiB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 546 transmembrane" amino acids 20 to 39 (20 residues), see Phobius details PF02470: MlaD" amino acids 43 to 134 (92 residues), 48 bits, see alignment E=5.9e-17 amino acids 158 to 218 (61 residues), 35 bits, see alignment E=7.2e-13 amino acids 288 to 389 (102 residues), 32.4 bits, see alignment E=4.6e-12

Best Hits

Swiss-Prot: 67% identical to PQIB_ECOLI: Intermembrane transport protein PqiB (pqiB) from Escherichia coli (strain K12)

KEGG orthology group: K06192, paraquat-inducible protein B (inferred from 96% identity to pwa:Pecwa_2067)

Predicted SEED Role

"Paraquat-inducible protein B" in subsystem Oxidative stress

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (546 amino acids)

>HER17_RS11975 intermembrane transport protein PqiB (Pectobacterium carotovorum WPP14)
MAKDNHAIADVETIKRWSPVWIVPIVTVLIGAWILFYHFSHQGPQITLITSNAEGIEAGK
TTIKSRSVDVGIVESVVLSDDLHQVEIKARLHDGMGKLLKQDSAFWVVKPQIGREGVSGL
GTLLSGAYIELQPGASKNDKSEFTLLDAPPLASPDAKGIRVILDSEQSGQLNAGDPVLFR
GYRVGSVETSEFDPKARKMRYQLFISAPYDGLVTTNVRFWKDSGVAFDMSAQGMRVEMGS
LTTLFSGGVSFDVPAGWELGDAAKTMAQYRLFDSQRSIQDSLYTEYKEYLLFFSESIRGL
QAGAPVEFRGIRLGTVAEAPFFPRNMKQELDDDYRIPVLIRIEPDRFEKKIGGSFDFEQH
LKQAQSLGLRASMKSANLLTGALYIDLDFYPKEKVDKQLFVLDGYPVLPTIDGGLSQIQQ
KLMAVLDKVNNLPLNPMVNEATKTLTESQATLREMQKTLATLNKLTSSKAMQDLPEDMQK
TLLELNRSMKGFQPGSPAYNKMVADMQRLDLVLRELQPVLRTLNEKSNALVFEASGSQDP
QPKRAK