Protein Info for HER17_RS11660 in Pectobacterium carotovorum WPP14

Annotation: spermidine/putrescine ABC transporter ATP-binding protein PotA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 371 PF00005: ABC_tran" amino acids 28 to 168 (141 residues), 127.7 bits, see alignment E=7.7e-41 TIGR01187: polyamine ABC transporter, ATP-binding protein" amino acids 41 to 365 (325 residues), 521 bits, see alignment E=5.7e-161 PF08402: TOBE_2" amino acids 285 to 365 (81 residues), 31.7 bits, see alignment E=2.1e-11

Best Hits

Swiss-Prot: 96% identical to POTA_PECAS: Spermidine/putrescine import ATP-binding protein PotA (potA) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K11072, spermidine/putrescine transport system ATP-binding protein [EC: 3.6.3.31] (inferred from 98% identity to pct:PC1_1862)

MetaCyc: 80% identical to spermidine preferential ABC transporter ATP binding subunit (Escherichia coli K-12 substr. MG1655)
ABC-25-RXN [EC: 7.6.2.11, 7.6.2.16]; 7.6.2.11 [EC: 7.6.2.11, 7.6.2.16]

Predicted SEED Role

"Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1)" in subsystem Polyamine Metabolism (TC 3.A.1.11.1)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.31

Use Curated BLAST to search for 3.6.3.31 or 7.6.2.11 or 7.6.2.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (371 amino acids)

>HER17_RS11660 spermidine/putrescine ABC transporter ATP-binding protein PotA (Pectobacterium carotovorum WPP14)
MKENSLTVPVVELLNVHKGFDGKDIISHFNLTINNGEFLTILGPSGCGKTTVLRLIAGLE
TVDSGNVMLEKQDITHQPAEQRHVNTVFQSYALFPHLSVFENVAFGLRMQKTPAAEIAPR
VIEALKMVQLEELAQRRPHQLSGGQQQRVAIARAVVNQPKVLLLDESLSALDYKLRKQMQ
NELKALQRKLGITFIFVTHDQEEALTMSDRIVVMRDGRIEQDGTPREIYEEPKNLFVASF
IGEINIFDAVVLELLNEQRVRAQVEGHDCVITVSFPVNVGQHLNVLLRPEDLRVEELGDG
MQAEGMVGYVRERNYKGMTLESNIELENGKMVMVSEFFNEDDPDFDHSLNQKVDVTWVES
WEVVLHDEEVA