Protein Info for HER17_RS11625 in Pectobacterium carotovorum WPP14

Annotation: tRNA 2-thiouridine(34) synthase MnmA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 368 PF02540: NAD_synthase" amino acids 4 to 76 (73 residues), 32 bits, see alignment E=2.6e-11 PF02568: ThiI" amino acids 5 to 76 (72 residues), 27.2 bits, see alignment E=9.9e-10 TIGR00420: tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase" amino acids 7 to 362 (356 residues), 520.8 bits, see alignment E=7.1e-161 PF00733: Asn_synthase" amino acids 7 to 186 (180 residues), 23.2 bits, see alignment E=1.8e-08 PF03054: tRNA_Me_trans" amino acids 7 to 203 (197 residues), 284.9 bits, see alignment E=1.2e-88 PF20259: tRNA_Me_trans_M" amino acids 209 to 277 (69 residues), 80.4 bits, see alignment E=2e-26 PF20258: tRNA_Me_trans_C" amino acids 288 to 362 (75 residues), 93.3 bits, see alignment E=3.3e-30

Best Hits

Swiss-Prot: 100% identical to MNMA_PECCP: tRNA-specific 2-thiouridylase MnmA (mnmA) from Pectobacterium carotovorum subsp. carotovorum (strain PC1)

KEGG orthology group: K00566, tRNA-specific 2-thiouridylase [EC: 2.8.1.-] (inferred from 100% identity to eca:ECA2442)

MetaCyc: 87% identical to tRNA-specific 2-thiouridylase (Escherichia coli K-12 substr. MG1655)
RXN0-2023 [EC: 2.8.1.13]

Predicted SEED Role

"tRNA-specific 2-thiouridylase MnmA"

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 2.8.1.-

Use Curated BLAST to search for 2.8.1.- or 2.8.1.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (368 amino acids)

>HER17_RS11625 tRNA 2-thiouridine(34) synthase MnmA (Pectobacterium carotovorum WPP14)
MSDNSQKKVIVGMSGGVDSSVSAYLLQQQGYHVEGLFMKNWEEDDDTEYCSAATDLADAQ
AVCDKLGIELHTVNFAAEYWDNVFELFLEEYKAGRTPNPDILCNKEIKFKAFLEFAAEDL
GADYIATGHYVRRHDVDGKSRLLRGMDGNKDQSYFLYTLSHEQIAQSLFPVGELEKPQVR
KIAEELELATAKKKDSTGICFIGERKFTDFLARYLPAQPGPILSVDDNKPMGQHQGLMYH
TLGQRKGLGIGGVKDGGEDPWYVVDKDVANNILYVAQGHEHPRLMSYGLIAQQLHWVDRQ
PLTAELRCTVKTRYRQADIPCTVTPLGDDRITVRFDEPVAAVTPGQSAVFYLDDVCLGGG
IIEERLQE