Protein Info for HER17_RS11325 in Pectobacterium carotovorum WPP14

Annotation: PTS mannose transporter subunit IIAB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 322 signal peptide" amino acids 1 to 15 (15 residues), see Phobius details TIGR00824: PTS system, mannose/fructose/sorbose family, IIA component" amino acids 3 to 116 (114 residues), 149.4 bits, see alignment E=4.4e-48 PF03610: EIIA-man" amino acids 5 to 116 (112 residues), 106.7 bits, see alignment E=8.1e-35 PF03830: PTSIIB_sorb" amino acids 163 to 309 (147 residues), 183.9 bits, see alignment E=2.2e-58 TIGR00854: PTS system, mannose/fructose/sorbose family, IIB component" amino acids 163 to 310 (148 residues), 188.7 bits, see alignment E=6.5e-60

Best Hits

Swiss-Prot: 74% identical to PTNAB_SHIFL: PTS system mannose-specific EIIAB component (manX) from Shigella flexneri

KEGG orthology group: K02793, PTS system, mannose-specific IIA component [EC: 2.7.1.69] K02794, PTS system, mannose-specific IIB component [EC: 2.7.1.69] (inferred from 96% identity to eca:ECA2385)

MetaCyc: 74% identical to mannose-specific PTS enzyme IIAB component (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-157 [EC: 2.7.1.199]; 2.7.1.199 [EC: 2.7.1.199]; TRANS-RXN-167 [EC: 2.7.1.199, 2.7.1.193]; TRANS-RXN-165 [EC: 2.7.1.199, 2.7.1.193, 2.7.1.191]; 2.7.1.191 [EC: 2.7.1.199, 2.7.1.193, 2.7.1.191]

Predicted SEED Role

"PTS system, mannose-specific IIA component (EC 2.7.1.69) / PTS system, mannose-specific IIB component (EC 2.7.1.69)" in subsystem Mannose Metabolism or Sialic Acid Metabolism (EC 2.7.1.69)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69

Use Curated BLAST to search for 2.7.1.191 or 2.7.1.193 or 2.7.1.199 or 2.7.1.69

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (322 amino acids)

>HER17_RS11325 PTS mannose transporter subunit IIAB (Pectobacterium carotovorum WPP14)
MSIAIMLCTHGATAGPLLKTAEMILGEQSNVAWIDFVPGENAETLIEKYQEKLTQLDTSQ
GVLFLVDTWGGSPFNAASRLVTHKENHDVIAGVNIPMLAETFMARDDNPSFSDLVSIALE
AGRDGVKALKHQEAAKAASPSSPPPPKTNAIPTPAGPGEHMKIGLARIDDRLIHGQVATR
WTKETNVSRIIVVSDEVAADHVRKTLLTQVAPPGVTAHVVDVAKAVRVYDNPKYAADRVM
LLFTNPTDVLTLIENGVNITSVNIGGMAFKQGKTQVNNAVSIDEKDIAAFRELDKRGIEL
EVRKVSTDSRIKMMDLINKMPR