Protein Info for HER17_RS09885 in Pectobacterium carotovorum WPP14

Annotation: NAD-dependent succinate-semialdehyde dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00171: Aldedh
  • amino acids 25 to 483 (459 residues), 569.3 bits, E=2.8e-175
• TIGR01780: succinate-semialdehyde dehydrogenase
  • amino acids 36 to 482 (447 residues), 731 bits, E=2.6e-224

Best Hits

Swiss-Prot: 65% identical to GABD_ECOLI: Succinate-semialdehyde dehydrogenase [NADP(+)] GabD (gabD) from Escherichia coli (strain K12)

KEGG orthology group: K00135, succinate-semialdehyde dehydrogenase (NADP+) [EC: 1.2.1.16] (inferred from 96% identity to eca:ECA2054)

MetaCyc: 69% identical to NAD(P)+-dependent succinate semialdehyde dehydrogenase monomer (Cupriavidus necator H16)
Succinate-semialdehyde dehydrogenase (NAD(P)(+)). [EC: 1.2.1.16]; RXN-10823 [EC: 1.2.1.16]

Predicted SEED Role

"Aldehyde dehydrogenase B (EC 1.2.1.22)" in subsystem Glycerolipid and Glycerophospholipid Metabolism in Bacteria or Methylglyoxal Metabolism (EC 1.2.1.22)

MetaCyc Pathways

• 4-aminobutanoate degradation III (2/2 steps found)
• L-lactaldehyde degradation (aerobic) (2/2 steps found)
• superpathway of fucose and rhamnose degradation (9/12 steps found)
• methylglyoxal degradation IV (2/3 steps found)
• methylglyoxal degradation V (2/3 steps found)
• homotaurine degradation (1/2 steps found)
• superpathway of methylglyoxal degradation (5/8 steps found)
• lactate biosynthesis (archaea) (2/5 steps found)
• L-rhamnose degradation II (2/8 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Glutamate metabolism
• Pyruvate metabolism
• Tyrosine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.16, 1.2.1.22

Use Curated BLAST to search for 1.2.1.16 or 1.2.1.22

Protein Sequence (490 amino acids)

>HER17_RS09885 NAD-dependent succinate-semialdehyde dehydrogenase (Pectobacterium carotovorum WPP14)
MQLKSSISLKQQTLFRQHCLIDGEWQDSQNGERLNVTNPATDAVLGSVPLVTVSQTEQAI
AAAELALVEWRKKTGKERAALLQAWARLIMDNQEDLAVLLTAEQGKPLAEAWGEIAYATS
FIDWFAEEAKRIEGSVLQSPQGQQRLLVIKQPIGVCAAITPWNFPAAMITRKVGPALAAG
CTMIVKPAEQTPFTALALAELAQQAGIPRGVLQVIVGDAQSVGKVLCDSPVVRKLSFTGS
TEVGRILMAQSAPTVKKLSLELGGNAPFIVFDDADVDQAVKGILASKFRNSGQTCVCANR
IYVQNGIYPTLVERLVEEVRKLNVGDGTQPGVTQGPLIDADAVSKVEQHIADALSHGATL
LLGGKRHELGGTFFTPTIVGGVTREMRFAREETFGPVAPLFRFSDEAEAIAMANDTEFGL
AAYVYTRDAVRQWTVPEALDYGMVGINTGLISNEVAPFGGVKQSGLGREGSRFGIEDYLE
MKYLCVDLSR