Protein Info for HER17_RS09655 in Pectobacterium carotovorum WPP14

Annotation: FMN-dependent NADH-azoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 201 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details PF02525: Flavodoxin_2" amino acids 2 to 197 (196 residues), 179 bits, see alignment E=9.3e-57 PF03358: FMN_red" amino acids 3 to 174 (172 residues), 44.7 bits, see alignment E=1.1e-15

Best Hits

Swiss-Prot: 97% identical to AZOR_PECAS: FMN-dependent NADH-azoreductase (azoR) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K01118, FMN-dependent NADH-azoreductase [EC: 1.7.-.-] (inferred from 97% identity to eca:ECA2005)

MetaCyc: 77% identical to FMN dependent NADH:quinone oxidoreductase (Escherichia coli K-12 substr. MG1655)
RXN0-5375 [EC: 1.7.1.17]; 1.6.5.- [EC: 1.7.1.17]

Predicted SEED Role

"FMN-dependent NADH-azoreductase"

Isozymes

Compare fitness of predicted isozymes for: 1.7.-.-

Use Curated BLAST to search for 1.7.-.- or 1.7.1.17

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (201 amino acids)

>HER17_RS09655 FMN-dependent NADH-azoreductase (Pectobacterium carotovorum WPP14)
MSKVLVLKSSILAGYSQSNQLADHFTAEWQSAHPNDSITVRDLAAQPIPVLDGELVGALR
PSDAALTPRQQEALKLSDDLIAELQAHDVIVIAAPMYNFNIPTQLKNYFDLVARAGVTFR
YTEQGPEGLVKGKRAIVLTSRGGIHQGTPTDLLEPYLRVFLGFLGLTDLEFVFAEGYGYG
PDVAQKATETAKTQLSQLVTA