Protein Info for HER17_RS09495 in Pectobacterium carotovorum WPP14

Annotation: peptide ABC transporter substrate-binding protein SapA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 562 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF00496: SBP_bac_5" amino acids 83 to 460 (378 residues), 297.7 bits, see alignment E=6.9e-93

Best Hits

Swiss-Prot: 75% identical to SAPA_ECOLI: Probable ABC transporter periplasmic-binding protein SapA (sapA) from Escherichia coli (strain K12)

KEGG orthology group: K02035, peptide/nickel transport system substrate-binding protein (inferred from 98% identity to pct:PC1_2342)

Predicted SEED Role

"Peptide transport periplasmic protein sapA (TC 3.A.1.5.5)" in subsystem ABC transporter peptide (TC 3.A.1.5.5) (TC 3.A.1.5.5)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (562 amino acids)

>HER17_RS09495 peptide ABC transporter substrate-binding protein SapA (Pectobacterium carotovorum WPP14)
MFGKTCFALALTWLALPALAAPPSVPTPPAPLENIRQSGFVYCVNDVLNTFNPQMARSGV
TIDTLAAQLYDRLLDVDPYTYRLMPELAQRWEVLDNGSTYRFYLRRDVPFQHTAWFSPTR
NMNADDVIFSFQRMLDEKHPYHDVNGGEYPYFDSLQFADSVQSIRKLGEYSIEIRLNSPD
ASFLWHLATHYAPILSAEYAQRLTKEDKKELLDREPVGTGPYLLNEYRNGQYIRLTRNSD
YWRGLPRMRQVVVDLGSGGTGRLSKLLTGECDVLAYPAASQLTILRNDPRLRLSLRPGMN
VAYLAFNVRKPPLDDRRVREAIALAINNDRLMQSIYYGTAETAASILPRASWAYDNESQI
TEYNPQKARQILQELGLTNLNLRLWVPSASQSYNPSPLKTAELIQADLAQIGVTVTIVPV
EGRFQEARLMELSHDLTLAGWATDSNDPDSFFRPLLSCAAIRSQSNYAHWCDPTFDEVLQ
NALSSQQLSKRIDYYQQAQRILAEQLPVLPLASSLRLLAYRYDMKGLVLSPFGNASFAGV
FREDQQAQQKPSAPETVEEAQP