Protein Info for HER17_RS09105 in Pectobacterium carotovorum WPP14

Annotation: ferredoxin-type protein NapF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 178 TIGR00402: ferredoxin-type protein NapF" amino acids 6 to 155 (150 residues), 159.4 bits, see alignment E=2.9e-51 PF12838: Fer4_7" amino acids 35 to 80 (46 residues), 32.9 bits, see alignment E=3.3e-11 amino acids 108 to 154 (47 residues), 37.9 bits, see alignment E=8.9e-13 PF13187: Fer4_9" amino acids 35 to 80 (46 residues), 32.4 bits, see alignment E=3.5e-11 amino acids 108 to 155 (48 residues), 28.8 bits, see alignment E=4.5e-10 PF12800: Fer4_4" amino acids 36 to 49 (14 residues), 14.4 bits, see alignment (E = 1.9e-05) amino acids 138 to 153 (16 residues), 18.1 bits, see alignment (E = 1.2e-06) PF12837: Fer4_6" amino acids 133 to 155 (23 residues), 26.1 bits, see alignment (E = 2.8e-09) PF00037: Fer4" amino acids 133 to 156 (24 residues), 28.4 bits, see alignment (E = 4.7e-10)

Best Hits

Swiss-Prot: 52% identical to NAPF_ECO57: Ferredoxin-type protein NapF (napF) from Escherichia coli O157:H7

KEGG orthology group: K02572, ferredoxin-type protein NapF (inferred from 92% identity to eca:ECA1898)

Predicted SEED Role

"Ferredoxin-type protein NapF (periplasmic nitrate reductase)" in subsystem Nitrate and nitrite ammonification

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (178 amino acids)

>HER17_RS09105 ferredoxin-type protein NapF (Pectobacterium carotovorum WPP14)
MTNLSRRSLLTGGLQRADNALRPPWSGAESHFLSQCTRCNVCVDACGAGIIQRGSGGFPT
VDFQRGECTFCYDCARACPQALFAESHTSPWEYHLTIQDACLSLHQVECRSCQDACETGA
IRFRPTIGRVAAPTIDDDACTTCGACISGCPVGAISMKKITAGYHTAPARLPTQQENR