Protein Info for HER17_RS07355 in Pectobacterium carotovorum WPP14

Annotation: cupin domain-containing protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03079: ARD
  • amino acids 17 to 163 (147 residues), 98.6 bits, E=6.7e-32
• PF00190: Cupin_1
  • amino acids 96 to 169 (74 residues), 23.2 bits, E=6.6e-09

Best Hits

Swiss-Prot: 95% identical to MTND1_PECAS: Acireductone dioxygenase 1 (mtnD1) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K08967, 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase [EC: 1.13.11.53 1.13.11.54] (inferred from 95% identity to pwa:Pecwa_1549)

MetaCyc: 52% identical to acireductone dioxygenase (Klebsiella oxytoca)
Acireductone dioxygenase (Fe(2+)-requiring). [EC: 1.13.11.54]; Acireductone dioxygenase (Ni(2+)-requiring). [EC: 1.13.11.54, 1.13.11.53]

Predicted SEED Role

"1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (EC 1.13.11.54)" in subsystem Methionine Salvage (EC 1.13.11.54)

MetaCyc Pathways

• L-methionine salvage cycle I (bacteria and plants) (11/12 steps found)
• L-methionine salvage cycle III (10/11 steps found)
• L-methionine salvage cycle II (plants) (9/11 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation I (6/7 steps found)
• 3-methylthiopropanoate biosynthesis (1/1 steps found)

KEGG Metabolic Maps

• Methionine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.13.11.53, 1.13.11.54

Use Curated BLAST to search for 1.13.11.53 or 1.13.11.54

Protein Sequence (173 amino acids)

>HER17_RS07355 cupin domain-containing protein (Pectobacterium carotovorum WPP14)
MTTLSIYHRPHITQPVQQLTTFNDIASLLASAGIQLEHWQTEAPPLDASSETILTQYRAD
IERLKQQEGYTSADVISLTPDHAERLALREKFLQEHTHSEDEVRFFVRGSGSFFVPIGEQ
VFQLTCEAGDLLRVPANTPHWFDCGEFPDFVAIRIFTNPEGWVGHFTGRETFH