Protein Info for HER17_RS07330 in Pectobacterium carotovorum WPP14

Annotation: nitrite reductase large subunit NirB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1395 PF07992: Pyr_redox_2" amino acids 4 to 280 (277 residues), 186.4 bits, see alignment E=5.3e-58 amino acids 422 to 702 (281 residues), 181.1 bits, see alignment E=2.1e-56 PF00070: Pyr_redox" amino acids 148 to 227 (80 residues), 60.1 bits, see alignment 1.6e-19 amino acids 564 to 634 (71 residues), 57.1 bits, see alignment 1.4e-18 PF18267: Rubredoxin_C" amino acids 318 to 385 (68 residues), 48.4 bits, see alignment 4.7e-16 amino acids 735 to 803 (69 residues), 44.1 bits, see alignment 1e-14 TIGR02374: nitrite reductase [NAD(P)H], large subunit" amino acids 422 to 1215 (794 residues), 1120.9 bits, see alignment E=0 PF04324: Fer2_BFD" amino acids 839 to 886 (48 residues), 40.8 bits, see alignment (E = 1.5e-13) PF03460: NIR_SIR_ferr" amino acids 975 to 1036 (62 residues), 47.4 bits, see alignment (E = 8.5e-16) PF01077: NIR_SIR" amino acids 1047 to 1183 (137 residues), 86 bits, see alignment E=1.3e-27 TIGR02378: nitrite reductase [NAD(P)H], small subunit" amino acids 1269 to 1373 (105 residues), 100.1 bits, see alignment 7.2e-33 PF13806: Rieske_2" amino acids 1269 to 1373 (105 residues), 106.1 bits, see alignment 5.8e-34

Best Hits

Predicted SEED Role

"Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4) / Nitrite reductase [NAD(P)H] small subunit (EC 1.7.1.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.1.4

Use Curated BLAST to search for 1.7.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1395 amino acids)

>HER17_RS07330 nitrite reductase large subunit NirB (Pectobacterium carotovorum WPP14)
MKPHLIVIGNGMASARFVDVLRQLAATRYRITVIGDEPRASYNRILLSPVLSGEKAFTDT
LLTPATLDDDAALPVNYLLGERVTHIDRQQREVTTTQRQLHYDHLVLATGSTPFMPPMPG
IDLAGVCGFRTLDDVELMLTTIGQSVPAVVIGGGLLGIEAAAALKLRGADVTLLHRVPVL
MERQLDATASDLLCNSLRERGIACETDVQVVALHGDERGVTAVALADGRTIPTGLVVVTA
GVIPASQLARECGLPCNRGVLVDGQLQTADPYISAIGECCELNGEIFGLVAPCFAHATLV
AQRLAGHTPKDYQREQAATRLKVTGIGVVSGGDINVTPDDEVYTLFDPQTQHYRRLLVRD
GRLSGVLLYGDTDDSQRLLATMDSTTEGEMIPPASLLFGLSSPDSDPQPEAVRIPVMSKP
ILVVVGHGMVGHYFLEQLVERDLHQHYHIVVFGEERHEAYDRVHLSEYFSGRSAASLSLV
KDGFFAESGIELRSASEIVAIDRERQCVCDAQGRETAYDKLVLATGSYAFVPPIPGNTRP
GCLVYRTLDDLDAIAAQAKKGKSGVVIGGGLLGLEAANALRQLGLDTHVVEFAPRLMAVQ
LDDGGATMLRRKIEALGVQIHLSKETREITDGEQALHRLCFADGSILETDLVLFSAGIRP
RDKLADSCDLEKGPRGGIVIDDSCQTSDDAIFAIGECALWKGQIFGLVAPGYQMARSVAD
TLAQRDTPFTGADMSTKLKLLGVEVASIGDAHGRTAGSQSYQWTDGPNEVYKKIVVSADG
KRLLGAVLIGDSSDYSTLLQMMLNDMPLPKQPEGLILPARSGDAPKGLGVAALPASAQIC
SCHNVSKGDISAAVAGGCGELGALKTCTKAGTGCGGCVPLLKQVMEYELTQLGVEVKKDI
CEHFAYSRQELYHLIRVHEIRSFDSLLERYGHGLGCEVCKPLVGSMLASCWNDYLLQPQH
LPLQDTNDRFFANIQKDGTYSVVPRVPAGEITPEGLIAIGQVAQRYNLYTKITGGQRVDL
FGARLEQLPEIWRELIDAGFETGHAYGKSLRTVKSCVGSTWCRYGVQDSTGLAIQLEHRY
KGLRSPHKVKMAVSGCTRECAEAQSKDIGVIATDKGWNLYVCGNGGMKPRHADLFASDLD
TETLLRTIDRVLMFYIRTGDRLQRTSTWMDNLEGGIDYLRQVILEDSLNIGEELDKEMQR
VVDAYQCEWQTTLESPERLALFRGFLNSDSPDEAVVMVPERGQIRPAQDHEKAVSAEPAV
LKPAAHEAEWVQVATLGDIPRHAGMAARLGQQQIALFHLPGSEQQVYALENHEPGSGANV
LSRGLLGDVAGEPVVISPLYKKRFKLRDGVSPDDSALYVRAWPVRVEDDEIWVCRQPLAV
PESVSLAETAMAKAS