Protein Info for HER17_RS07195 in Pectobacterium carotovorum WPP14

Annotation: alpha,alpha-phosphotrehalase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 563 TIGR02403: alpha,alpha-phosphotrehalase" amino acids 7 to 550 (544 residues), 846 bits, see alignment E=6.9e-259 PF00128: Alpha-amylase" amino acids 30 to 371 (342 residues), 431.5 bits, see alignment E=4.4e-133 PF11941: DUF3459" amino acids 459 to 538 (80 residues), 33.7 bits, see alignment E=6e-12 PF16657: Malt_amylase_C" amino acids 475 to 546 (72 residues), 31.4 bits, see alignment E=2.5e-11

Best Hits

Swiss-Prot: 72% identical to TREC_ECOLI: Trehalose-6-phosphate hydrolase (treC) from Escherichia coli (strain K12)

KEGG orthology group: K01226, trehalose-6-phosphate hydrolase [EC: 3.2.1.93] (inferred from 94% identity to pct:PC1_2758)

MetaCyc: 72% identical to trehalose-6-phosphate hydrolase (Escherichia coli K-12 substr. MG1655)
Alpha,alpha-phosphotrehalase. [EC: 3.2.1.93]

Predicted SEED Role

"Trehalose-6-phosphate hydrolase (EC 3.2.1.93)" in subsystem Trehalose Uptake and Utilization (EC 3.2.1.93)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.93

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (563 amino acids)

>HER17_RS07195 alpha,alpha-phosphotrehalase (Pectobacterium carotovorum WPP14)
MNASLPWWQNGVIYQIYPKSFQDSTGNGIGDIAGITARLDYLQQLGIDAIWLTPVYLSPQ
VDNGYDVADYCAIDPAYGTMADMETLIAEAHQRRIRVVMDMVFNHTSTQHHWFQNAQDRR
SPYRHFYIWRDGNDGALPNNWRSKFGGPAWQWHEESKQYYLHLFATEQADLNWEHPRVRD
ELKQVCAFWADKGVDGLRLDVINLVSKQQDFPSDAQGDGRRFYTDGPLIHDYLQEFSRDV
FQPRGLMTVGEMSSTSLDHCRRYAALDGNELSMTFNFHHLKVDYPSGEKWTLAAPDFIQL
KQIFNHWQQGMHNHAWNALFWCNHDQPRIVSRFGDEGKFRVQSAKMLAMVLHGMQGTPYI
YQGEELGMTNPGYTRIEQYRDIESLNQFAEQRERGQPDEQTLAILASKSRDNGRTPMQWD
DSHHAGFTTGTPWITPCQNVDHINAAQALADKDSVFYTYQQLIAMRKALPLLTYGDYQDL
LPDHPNVWCYQRLWEGQRLLVLANLSKQTQRWQPPIDTHDRRWRLLFSNYSDAQPQPAIL
ALRPFEAIYWVQGMPEKEDKIQG