Protein Info for HER17_RS06550 in Pectobacterium carotovorum WPP14

Annotation: 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 130 signal peptide" amino acids 1 to 37 (37 residues), see Phobius details transmembrane" amino acids 46 to 70 (25 residues), see Phobius details amino acids 80 to 100 (21 residues), see Phobius details amino acids 106 to 123 (18 residues), see Phobius details PF00892: EamA" amino acids 47 to 123 (77 residues), 34.8 bits, see alignment E=8.9e-13

Best Hits

Swiss-Prot: 85% identical to ARNF_PECAS: Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnF (arnF) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K12963, undecaprenyl phosphate-alpha-L-ara4N flippase subunit ArnF (inferred from 85% identity to pwa:Pecwa_1356)

MetaCyc: 36% identical to undecaprenyl-phosphate-alpha-L-Ara4N flippase - ArnF subunit (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-276

Predicted SEED Role

"Polymyxin resistance protein PmrM" in subsystem Lipid A-Ara4N pathway ( Polymyxin resistance )

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (130 amino acids)

>HER17_RS06550 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnF (Pectobacterium carotovorum WPP14)
MAKRGYIWAIISIVLASTAQVLMKSGMLALPSISFAHWPSLSSLVAGWPAVAVLVGVICY
GLSMVCWFMVLRYLPLSRAYPLISLSYAVVYLAAVFLPWLNEPMSLRKTLGVLVILLGVW
LVSRNAQAAR