Protein Info for HER17_RS06200 in Pectobacterium carotovorum WPP14

Annotation: FMN-dependent NADH-azoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 206 PF02525: Flavodoxin_2" amino acids 1 to 199 (199 residues), 163.7 bits, see alignment E=4.5e-52 PF03358: FMN_red" amino acids 1 to 133 (133 residues), 36.8 bits, see alignment E=3e-13

Best Hits

Swiss-Prot: 55% identical to AZOR_RALSO: FMN-dependent NADH-azoreductase (azoR) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K01118, FMN-dependent NADH-azoreductase [EC: 1.7.-.-] (inferred from 92% identity to pct:PC1_2993)

Predicted SEED Role

"FMN-dependent NADH-azoreductase"

Isozymes

Compare fitness of predicted isozymes for: 1.7.-.-

Use Curated BLAST to search for 1.7.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (206 amino acids)

>HER17_RS06200 FMN-dependent NADH-azoreductase (Pectobacterium carotovorum WPP14)
MNILHIDSSILESGSVSRQISADVVEQLVKQHPQAVVAYRDVVKDEIRHLTGPIAAGFRQ
IASSSADEATLREHQLSEALVAEFLANDVIVIGAPMYNFSVSSQLKSWLDRLAQAGKTFK
YTEKGPVGLSGGRTVIVVSSRGGFYTTPPFLDMDFQERYLQGFFRFLGITDIHFIRAEGA
SKGDEIKHREIQNAQSSIHSVVTSIA