Protein Info for HER17_RS04640 in Pectobacterium carotovorum WPP14

Annotation: acyl-CoA dehydrogenase FadE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02771: Acyl-CoA_dh_N
  • amino acids 145 to 233 (89 residues), 66.6 bits, E=3.9e-22
• PF00441: Acyl-CoA_dh_1
  • amino acids 360 to 507 (148 residues), 48.2 bits, E=2e-16
• PF09317: ACDH_C
  • amino acids 514 to 792 (279 residues), 401.3 bits, E=4.6e-124

Best Hits

Swiss-Prot: 79% identical to FADE_YERPE: Acyl-coenzyme A dehydrogenase (fadE) from Yersinia pestis

KEGG orthology group: K06445, acyl-CoA dehydrogenase [EC: 1.3.99.-] (inferred from 97% identity to eca:ECA3474)

MetaCyc: 77% identical to acyl-CoA dehydrogenase (Escherichia coli K-12 substr. MG1655)
RXN-17775 [EC: 1.3.8.8]; 1.3.8.8 [EC: 1.3.8.8]; 1.3.8.8 [EC: 1.3.8.8]; 1.3.8.8 [EC: 1.3.8.8]; 1.3.8.8 [EC: 1.3.8.8]; 1.3.8.8 [EC: 1.3.8.8]; RXN-17796 [EC: 1.3.8.8, 1.3.8.7]; 1.3.8.- [EC: 1.3.8.8, 1.3.8.7]

Predicted SEED Role

"Butyryl-CoA dehydrogenase (EC 1.3.99.2)" in subsystem Acetyl-CoA fermentation to Butyrate or Anaerobic respiratory reductases or Butanol Biosynthesis or Isobutyryl-CoA to Propionyl-CoA Module or Isoleucine degradation or Valine degradation (EC 1.3.99.2)

MetaCyc Pathways

• oleate β-oxidation (29/35 steps found)
• fatty acid β-oxidation I (generic) (6/7 steps found)
• pyruvate fermentation to hexanol (engineered) (8/11 steps found)
• fatty acid salvage (4/6 steps found)
• pyruvate fermentation to butanol II (engineered) (4/6 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (19/27 steps found)
• adipate degradation (3/5 steps found)
• 2-methyl-branched fatty acid β-oxidation (8/14 steps found)
• (8E,10E)-dodeca-8,10-dienol biosynthesis (5/11 steps found)

KEGG Metabolic Maps

• 1- and 2-Methylnaphthalene degradation
• Benzoate degradation via CoA ligation
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Brassinosteroid biosynthesis
• Butanoate metabolism
• Caprolactam degradation
• Fatty acid metabolism
• Geraniol degradation
• Valine, leucine and isoleucine degradation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.8.7 or 1.3.8.8 or 1.3.99.- or 1.3.99.2

Protein Sequence (815 amino acids)

>HER17_RS04640 acyl-CoA dehydrogenase FadE (Pectobacterium carotovorum WPP14)
MVAVSILLLLIIIGAMFYHRLSLLLGSAILLAYFAAMSAMLLWPVWLMIPLVILLIPITV
PSLRQKLVSASALRMFQKVMPPMSNTEKEAIDAGTTWWEGDLFRGAPDWKKLHNYPRPQL
TAEEQAFIDGPVAEACRMANDFEITHERADIPPELWEYLKEHRFFAMIIKKQYGGLEFSA
YAQAQVLQKLAGVSSIVAITVGVPNSLGPGELLQHYGTDAQKDHYLPRLARGQEVPCFAL
TSPEAGSDAGSIPDTGIVCYGNWHGEQVVGMRLTWNKRYITLAPVATVLGLAFKLYDPDH
LLGDQDDVGITCALIPTDTDGVKIGRRHFPLNVPFQNGPTQGENIFVPLDYIIGGAKMAG
QGWRMLMECLSVGRGITLPSNSTGGLKSIALGIGAYAHIRRQFKISIGKMEGIEEPLARI
AGNAYVMDAAATLITSGIMQGEKPAVLSAIVKYHCTHRGQRSVMDAMDIAGGKGICLGPS
NFLARSYQGAPIAITVEGANILTRSMIIFGQGAIRCHPYVLEEMAAAQENDVSRFDRALV
GHIGHVGSNKVRSFWLGLTNGLLSRAPVNDKTRRYYQQLNRLSANLALLADVSMAVLGGS
LKRRERISARLGDILSQLYLASATLKRYEDEGRQKADLPLVHWGVQDSLHQAEQALDDLL
RNFPNRAVAGLLTFIIFPLGLRCAAPSDRLDHEVAKILQTPSETRSRLGRGQYLVASEHN
PIGLLEEALLDIMAAEPIYQRLSKASDKPLPFMRLDQLAEQALIEGQITAEEADILTKAE
ASRLRSINVDDFAFDALAANKPAPVPQPERKTEAA