Protein Info for HER17_RS04240 in Pectobacterium carotovorum WPP14
Annotation: assimilatory sulfite reductase (NADPH) hemoprotein subunit
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 97% identical to CYSI2_PECCP: Sulfite reductase [NADPH] hemoprotein beta-component 2 (cysI2) from Pectobacterium carotovorum subsp. carotovorum (strain PC1)
KEGG orthology group: K00381, sulfite reductase (NADPH) hemoprotein beta-component [EC: 1.8.1.2] (inferred from 98% identity to pwa:Pecwa_3526)MetaCyc: 83% identical to sulfite reductase, hemoprotein subunit (Escherichia coli K-12 substr. MG1655)
Sulfite reductase (NADPH). [EC: 1.8.1.2]
Predicted SEED Role
"Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2)" in subsystem Cysteine Biosynthesis (EC 1.8.1.2)
MetaCyc Pathways
- superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
- superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (10/10 steps found)
- superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
- assimilatory sulfate reduction I (4/4 steps found)
- assimilatory sulfate reduction III (3/3 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.8.1.2
Use Curated BLAST to search for 1.8.1.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (577 amino acids)
>HER17_RS04240 assimilatory sulfite reductase (NADPH) hemoprotein subunit (Pectobacterium carotovorum WPP14) MSEKYVFSEKHPGPLVVEGKLADAERMKTESNFLRGTIAEDLNDGLTGGFRGDNFLLIRF HGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVMTPEQWLRIDKFATENTIYGSIRI TNRQTFQYHGILKSNVKPVHQMLNSIGLDALATANDMNRNVLCTSNPIESELHQQAYEWA KKISEHLLPRTRAYAEIWMDQEKVATTDEEPILGSTYLPRKFKTTVVIPPQNDIDLHAND LNFVAIADNGRLVGFNVLVGGGLSIAHGDKETYPRTASELGYISIEHTLAIAEAVVTTQR DWGNRTNRKNAKTKYTLERVGVDNFKQEVEARAGVKFEAVRPYEFTGRGDRIGWVKGIDN KWHLTLFIENGRVLDYPGRPLKTGLAEIAKIHKGDFRLTANQNLIIAGVPARSKAKIDAL AREHGLIDDGVSEQRKNSMACVSFPTCPLAMAEAERFLPEFVTKVEGIMQQHGVGDEHIV LRVTGCPNGCGRAMLAEIGLVGKAVGRYNLHLGGNREGTRIPRMYRENINETEILAEMDR LIGLWAQDRQPNEGFGDFVIRTNIIKAVLDPARDFYD