Protein Info for HER17_RS02840 in Pectobacterium carotovorum WPP14

Annotation: molecular chaperone DnaK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 635 TIGR02350: chaperone protein DnaK" amino acids 3 to 601 (599 residues), 959.3 bits, see alignment E=3.5e-293 PF00012: HSP70" amino acids 4 to 600 (597 residues), 883.7 bits, see alignment E=5.4e-270 PF06723: MreB_Mbl" amino acids 114 to 376 (263 residues), 45.3 bits, see alignment E=5.4e-16

Best Hits

Swiss-Prot: 100% identical to DNAK_PECCP: Chaperone protein DnaK (dnaK) from Pectobacterium carotovorum subsp. carotovorum (strain PC1)

KEGG orthology group: K04043, molecular chaperone DnaK (inferred from 99% identity to eca:ECA3882)

MetaCyc: 89% identical to chaperone protein DnaK (Escherichia coli K-12 substr. MG1655)
Non-chaperonin molecular chaperone ATPase. [EC: 3.6.4.10, 5.6.1.7]

Predicted SEED Role

"Chaperone protein DnaK" in subsystem Heat shock dnaK gene cluster extended or Protein chaperones

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.4.10 or 5.6.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (635 amino acids)

>HER17_RS02840 molecular chaperone DnaK (Pectobacterium carotovorum WPP14)
MGKIIGIDLGTTNSCVAIIDGTQVKVLENSEGDRTTPSIIAYTQDGETLVGQPAKRQAVT
NPKNTLFAIKRLIGRRFKDEEVQRDANIMPYKIIAADNGDAWLEVKDQKMAPPQISAEVL
KKMKKTAEDYLGETITEAVITVPAYFNDAQRQATKDAGRIAGLEVKRIINEPTAAALAYG
LDKEVGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEVLATNGDTHLGGEDFDSRMINYL
VDEFKKEQGFDLRNDPLAMQRLKEAAEKAKIELSSAQQTDVNLPYITADATGPKHLNIKV
TRAKLESLVEELVNRSLEPLKVALQDAGLSVSEIQDVILVGGQTRMPLVQKKVADFFGKE
PRKDVNPDEAVAIGAAVQGGVLSGDVKDVLLLDVTPLSLGIETMGGVMTALIAKNTTIPT
KHSQVFSTAEDNQSAVTIHVLQGERKRAHDNKSLGQFNLDGIQAAPRGMPQIEVTFDIDA
DGILHVSAKDKNSGREQKITIKASSGLNEEEIQKMVRDAEANAESDRKFEELVQARNQGD
HLLHSTRKQLEEVGDKLAADDKTAIDDALKALESALKGEDKAEIEAKIQALVQVSGKLLE
ASQPQPGAEGAADDASARRDDDVVDAEFEEVKDKK