Protein Info for HER17_RS02640 in Pectobacterium carotovorum WPP14

Annotation: 16S rRNA (uracil(1498)-N(3))-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00046: RNA methyltransferase, RsmE family
  • amino acids 4 to 243 (240 residues), 276.2 bits, E=1e-86
• PF20260: PUA_4
  • amino acids 21 to 67 (47 residues), 52.9 bits, 3.2e-18
• PF04452: Methyltrans_RNA
  • amino acids 75 to 237 (163 residues), 183.2 bits, E=2.8e-58

Best Hits

Swiss-Prot: 81% identical to RSME_DICD3: Ribosomal RNA small subunit methyltransferase E (rsmE) from Dickeya dadantii (strain 3937)

KEGG orthology group: K09761, ribosomal RNA small subunit methyltransferase E [EC: 2.1.1.-] (inferred from 95% identity to pct:PC1_3710)

MetaCyc: 77% identical to 16S rRNA m3U1498 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN-11598 [EC: 2.1.1.193]

Predicted SEED Role

"Ribosomal RNA small subunit methyltransferase E (EC 2.1.1.-)" in subsystem Heat shock dnaK gene cluster extended (EC 2.1.1.-)

KEGG Metabolic Maps

• Alkaloid biosynthesis I
• Anthocyanin biosynthesis
• Benzoxazinone biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway
• Carotenoid biosynthesis - General
• Flavonoid biosynthesis
• Histidine metabolism
• Insect hormone biosynthesis
• Naphthalene and anthracene degradation
• Phenylpropanoid biosynthesis
• Porphyrin and chlorophyll metabolism
• Tryptophan metabolism
• Tyrosine metabolism
• Ubiquinone and menaquinone biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.- or 2.1.1.193

Protein Sequence (244 amino acids)

>HER17_RS02640 16S rRNA (uracil(1498)-N(3))-methyltransferase (Pectobacterium carotovorum WPP14)
MRVPRIFHPETLSLNGGEAELSDDAANHVGRVLRMTTGQSLQLFDGSNHVFDAEIIAAGK
KSVRVRFTAGKLEDKESPLHLHLGQVMSRGEKMEFTIQKSIELGVNVITPLLSERCGVKL
DAERMEKKISQWQKIAVAACEQSGRNCVPLVRPAMTLEAWCAEQDDALKLNLHPRATQSI
NTLPLPVARVRLLIGPEGGLTAGEITMTSEHGFTDILLGPRVLRTETTALTAMTALQVRF
GDLG