Protein Info for HER17_RS02610 in Pectobacterium carotovorum WPP14

Annotation: adenylosuccinate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00184: adenylosuccinate synthase
  • amino acids 6 to 430 (425 residues), 669.4 bits, E=1e-205
• PF00709: Adenylsucc_synt
  • amino acids 6 to 424 (419 residues), 610.9 bits, E=5.3e-188

Best Hits

Swiss-Prot: 99% identical to PURA_PECCP: Adenylosuccinate synthetase (purA) from Pectobacterium carotovorum subsp. carotovorum (strain PC1)

KEGG orthology group: K01939, adenylosuccinate synthase [EC: 6.3.4.4] (inferred from 98% identity to pwa:Pecwa_3914)

MetaCyc: 89% identical to adenylosuccinate synthetase (Escherichia coli K-12 substr. MG1655)
Adenylosuccinate synthase. [EC: 6.3.4.4]

Predicted SEED Role

"Adenylosuccinate synthetase (EC 6.3.4.4)" in subsystem CBSS-262719.3.peg.410 or Purine conversions (EC 6.3.4.4)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (26/26 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotide salvage (13/14 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis II (7/7 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis I (5/5 steps found)
• adenosine ribonucleotides de novo biosynthesis (3/3 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Biosynthesis of alkaloids derived from histidine and purine
• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.4.4

Protein Sequence (432 amino acids)

>HER17_RS02610 adenylosuccinate synthase (Pectobacterium carotovorum WPP14)
MGKNVVVLGTQWGDEGKGKVVDLLTERAKYVVRYQGGHNAGHTLVINGEKTVLHLIPSGI
LRENVVSIIGNGVVLAPDALMKEMTELEARGVPVRERLLLSEACPLILPYHVALDNAREK
ARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAVKLKEIVEYHNFQLVNYYKAD
AVDYQKVLDDVLAIADILTAMVVDVSDLLYKAHLRGDFVMFEGAQGTLLDIDHGTYPYVT
SSNTTAGGVATGSGLGPRYVDYVLGIVKAYSTRVGAGPFPTELFEDVGEHLSQKGNEFGA
TTGRRRRTGWLDAVAVRRAVQINSLSGFCLTKLDVLDGLKEIKICVGYRLPNGSEVDTTP
LAAEGWEGLEPIYETMPGWSESTFGVKDHSKLPQAALNYIKRIEEITGVPIDIISTGPDR
SETMVLRDPFDA