Protein Info for HER17_RS01745 in Pectobacterium carotovorum WPP14

Annotation: peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 851 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF00912: Transgly" amino acids 54 to 230 (177 residues), 220.3 bits, see alignment E=1.9e-69 TIGR02074: penicillin-binding protein, 1A family" amino acids 65 to 785 (721 residues), 566.9 bits, see alignment E=2.5e-174 PF17092: PCB_OB" amino acids 316 to 425 (110 residues), 105.4 bits, see alignment E=4.5e-34 PF00905: Transpeptidase" amino acids 428 to 733 (306 residues), 88.4 bits, see alignment E=7.5e-29

Best Hits

Swiss-Prot: 78% identical to PBPA_ECOLI: Penicillin-binding protein 1A (mrcA) from Escherichia coli (strain K12)

KEGG orthology group: K05366, penicillin-binding protein 1A [EC: 2.4.1.- 3.4.-.-] (inferred from 97% identity to eca:ECA4099)

MetaCyc: 78% identical to peptidoglycan glycosyltransferase / peptidoglycan DD-transpeptidase MrcA (Escherichia coli K-12 substr. MG1655)
Serine-type D-Ala-D-Ala carboxypeptidase. [EC: 3.4.16.4]; 3.4.16.4 [EC: 3.4.16.4]; Peptidoglycan glycosyltransferase. [EC: 3.4.16.4, 2.4.1.129]

Predicted SEED Role

"Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-)" in subsystem Peptidoglycan Biosynthesis or Type IV pilus (EC 2.4.1.129, EC 3.4.-.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.-, 2.4.1.129, 3.4.-.-, 3.4.16.4

Use Curated BLAST to search for 2.4.1.- or 2.4.1.129 or 3.4.-.- or 3.4.16.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (851 amino acids)

>HER17_RS01745 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA (Pectobacterium carotovorum WPP14)
MKFVKYLFILAVSCILLGAASIYGLYRYIEPQLPDVATLKDVRLQTPMQVYSADGELIAQ
FGEKRRIPLKLDQIPPELVHAFIATEDSRFYDHHGVDPVGIIRAASIALTSGHASQGAST
ITQQLARNFFLSPERTLIRKIKEVFLAIRIEQLLTKDEILELYLNKIYLGYRAYGVGASA
QVYFGRPVDQLTLSEMAMIAGLPKAPSTFNPLYSYDRAVARRNVVLARMKDENYITQSQY
DAARNEKLVANYHAPDISFSAPYVAEMARQEMVKRYGEDAYNDGYKVYTTVTKKLQTAAQ
DALRNNILAYDMRHGYRGPSKVLWKVGEGVWDQAKMIAALKALPVYGPLSPAIVTSTTAD
NAIAILSDGSNIALPMAGMRWARAYRSDTQQGPTPKRVTDVVQAGQQVWVRKVNDDWWLA
QVPDVNSAIVSLNPKNGAIEALVGGFDFNQSKFNRATQALRQIGSNIKPFLYTAAMDKGL
TLATILNDVPITRWDAGAGSDWRPKNSPATYDGPIRLRQGLGQSKNVVMVRAMRAMGVDY
AADYLQRFGFPAQNIVHTESLALGAASFTPLQVVRGYAVMANGGYLVDPYLISKIESEAG
GTVFTATPKVVCDTCNLPLVYGDTPRSPVLATTNVEDVATSNESPPVGLPQPELEPVTPE
AAQQNAAQPYAPHVISTPLAFLIKDALNSNVFGEPGWMGTGWRAGRELKRRDIGGKTGTT
NSSKDAWFSGYGPNLVTSVWIGFDDHRRDLGASSASGVIKDQISGYEGGAKSAQPAWDDF
MKAALDGVPEQPLTPPPGIVSVVIDRSSGKLSNGGGNSRSEYFIDGTQPKEYEVHEVGTT
LMDNGQSEELF