Protein Info for HER17_RS01415 in Pectobacterium carotovorum WPP14

Annotation: sugar/pyridoxal phosphate phosphatase YigL

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 266 TIGR00099: Cof-like hydrolase" amino acids 4 to 260 (257 residues), 227.2 bits, see alignment E=2.6e-71 PF05116: S6PP" amino acids 4 to 241 (238 residues), 46.9 bits, see alignment E=4.1e-16 TIGR01484: HAD hydrolase, family IIB" amino acids 5 to 231 (227 residues), 93.6 bits, see alignment E=1.9e-30 PF08282: Hydrolase_3" amino acids 5 to 260 (256 residues), 222.6 bits, see alignment E=1.1e-69

Best Hits

Swiss-Prot: 78% identical to YIGL_ECOLI: Pyridoxal phosphate phosphatase YigL (yigL) from Escherichia coli (strain K12)

KEGG orthology group: K07024, (no description) (inferred from 98% identity to pwa:Pecwa_4150)

MetaCyc: 78% identical to phosphosugar phosphatase YigL (Escherichia coli K-12 substr. MG1655)
Pyridoxal phosphatase. [EC: 3.1.3.74]; Sugar-phosphatase. [EC: 3.1.3.74, 3.1.3.23]

Predicted SEED Role

"HMP-PP hydrolase (pyridoxal phosphatase) Cof, detected in genetic screen for thiamin metabolic genes (PMID:15292217)"

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.3.23 or 3.1.3.74

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (266 amino acids)

>HER17_RS01415 sugar/pyridoxal phosphate phosphatase YigL (Pectobacterium carotovorum WPP14)
MYHVVASDLDGTLLSPDHSLSPYAKETLRLLTEKGIHFVFATGRHHMDVAQIRDNLGISA
FMITSNGARVHNSQGELIFSHNLDQDIARDLYSVVHNNPDMLTHVYRDDEWFMNRPRAEE
ERFFKESIFKYKLFEPALLETDGVSKVFFTCDSHEQLLPLEEAINARWGDRVNVSFSTLT
CLEVMAGGVSKGHALEQVAKIIGFSLKECVAFGDGMNDYEMLSMAGKGCVMQNAHQRLKD
MLPDREVIGSNADSAVPNYLRELFLK