Protein Info for HER17_RS00495 in Pectobacterium carotovorum WPP14

Annotation: branched-chain amino acid ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 370 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF13433: Peripla_BP_5" amino acids 27 to 364 (338 residues), 67.3 bits, see alignment E=2e-22 PF13458: Peripla_BP_6" amino acids 27 to 363 (337 residues), 202.6 bits, see alignment E=2.3e-63 PF01094: ANF_receptor" amino acids 49 to 362 (314 residues), 97.1 bits, see alignment E=1.7e-31

Best Hits

Swiss-Prot: 76% identical to LIVK_ECOLI: Leucine-specific-binding protein (livK) from Escherichia coli (strain K12)

KEGG orthology group: K01999, branched-chain amino acid transport system substrate-binding protein (inferred from 98% identity to pct:PC1_0104)

MetaCyc: 76% identical to L-leucine/L-phenylalanine ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-35-RXN [EC: 7.4.2.2]; 7.4.2.2 [EC: 7.4.2.2]

Predicted SEED Role

"High-affinity leucine-specific transport system, periplasmic binding protein LivK (TC 3.A.1.4.1)" in subsystem ABC transporter branched-chain amino acid (TC 3.A.1.4.1) (TC 3.A.1.4.1)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.4.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (370 amino acids)

>HER17_RS00495 branched-chain amino acid ABC transporter substrate-binding protein (Pectobacterium carotovorum WPP14)
MKFSKGKVLLMGCMAAALSHAVSAADIKVAVVGAMSGPVAQYGDMEFIGARQAIEDINAK
GGVNGNKLVGVEYDDACDPKQAVAVANKVINDGIRYVIGHLCSSSTQPASDIYEEEGVIM
ITPAATNADLTTRGYKMVLRTTGLDSDQGPTSAKYIVETIKPQRIAVVHDKQQYGEGLAR
SVQDSLKKAGANVVLFEGVTAGDKDFSTLVARLKKENVDFVYFGGYYPEMGQILRQARQA
GMTTKFMGPEGVGNSSLSNIAGDASEGMLVTLPKRYDQVPANQPIVDALKAKKLDPTGPF
VWTTYAALQSLTTAMTRTGSDAPEKLVADLKANPVDTVMGPLSWDAKGDLKGFEFGVFEW
HKDGTSSAVK