Protein Info for HER17_RS00100 in Pectobacterium carotovorum WPP14
Annotation: nucleoside hydrolase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 41% identical to RIHA_ENT38: Pyrimidine-specific ribonucleoside hydrolase RihA (rihA) from Enterobacter sp. (strain 638)
KEGG orthology group: K01239, purine nucleosidase [EC: 3.2.2.1] (inferred from 96% identity to eca:ECA4424)Predicted SEED Role
"Inosine-uridine preferring nucleoside hydrolase (EC 3.2.2.1)" in subsystem Purine conversions or Queuosine-Archaeosine Biosynthesis (EC 3.2.2.1)
MetaCyc Pathways
- adenine and adenosine salvage II (2/2 steps found)
- guanine and guanosine salvage II (2/2 steps found)
- adenosine nucleotides degradation I (5/8 steps found)
- guanosine nucleotides degradation I (2/4 steps found)
- guanosine nucleotides degradation II (2/4 steps found)
- superpathway of guanosine nucleotides degradation (plants) (3/6 steps found)
- purine nucleotides degradation I (plants) (7/12 steps found)
- superpathway of purines degradation in plants (8/18 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 3.2.2.1
Use Curated BLAST to search for 3.2.2.1
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (317 amino acids)
>HER17_RS00100 nucleoside hydrolase (Pectobacterium carotovorum WPP14) MSALPIIIDCDPGIDDAIALLSAFVAPELDIRGICTVCGNQSLDKTVRNALQIVELGQRT DIPVFAGCHRPLLRDPIHGQFHGESGLGQTVLPAPQKQADAQHAVSFIIAQCKQALADGT PITLCTLGPLTNVATALRMAPEIADGIARIVMMGGAYREAGNRSLTSEFNMIADPQAAKV VFDSSIAIVALPLDVTHQVILTPELVARFIALSGRISAPLGEMMAFWDRNDIRRYGSRGG PLHDPLVIAWVLAPHCFTTEKASVYIEQESELCMGQTVADWYGKTDRQPNVDVVTGVDAK QVVELFADLLSRYREDV