Protein Info for HEPCGN_24910 in Escherichia coli ECOR38

Name: hemA
Annotation: glutamyl-tRNA reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 441 transmembrane" amino acids 12 to 31 (20 residues), see Phobius details TIGR01035: glutamyl-tRNA reductase" amino acids 27 to 441 (415 residues), 565.1 bits, see alignment E=4.7e-174 PF05201: GlutR_N" amino acids 29 to 179 (151 residues), 187.6 bits, see alignment E=1.8e-59 PF01488: Shikimate_DH" amino acids 195 to 329 (135 residues), 166.7 bits, see alignment E=4.7e-53 PF00745: GlutR_dimer" amino acids 343 to 439 (97 residues), 85.4 bits, see alignment E=4.5e-28

Best Hits

Swiss-Prot: 100% identical to HEM1_ECO7I: Glutamyl-tRNA reductase (hemA) from Escherichia coli O7:K1 (strain IAI39 / ExPEC)

KEGG orthology group: K02492, glutamyl-tRNA reductase [EC: 1.2.1.70] (inferred from 99% identity to sfv:SFV_1224)

MetaCyc: 99% identical to glutamyl-tRNA reductase (Escherichia coli K-12 substr. MG1655)
Glutamyl-tRNA reductase. [EC: 1.2.1.70]

Predicted SEED Role

"Glutamyl-tRNA reductase (EC 1.2.1.70)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis (EC 1.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.70

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (441 amino acids)

>HEPCGN_24910 glutamyl-tRNA reductase (Escherichia coli ECOR38)
LILRILYDASRLTLSTLVLFPADMTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLA
QPMVQGGVVLSTCNRTELYLSVEERDDLQEALIRWLCDYHNLNEDDLRNSLYWHQDNDAV
SHLMRVASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETD
IGASAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRE
RAQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLLV
DIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMAWLR
AQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAPTKSLQ
QAARDGDNERLNILRDSLGLE