Protein Info for HEPCGN_24870 in Escherichia coli ECOR38

Name: dhaR
Annotation: dihydroxyacetone kinase operon transcriptional regulator DhaR

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 639 PF01590: GAF" amino acids 56 to 187 (132 residues), 24.7 bits, see alignment E=8.9e-09 PF00989: PAS" amino acids 204 to 306 (103 residues), 52.9 bits, see alignment E=1.1e-17 PF00158: Sigma54_activat" amino acids 330 to 490 (161 residues), 124.3 bits, see alignment E=1.3e-39 PF14532: Sigma54_activ_2" amino acids 343 to 495 (153 residues), 66.4 bits, see alignment E=9.9e-22 PF02954: HTH_8" amino acids 588 to 627 (40 residues), 53.1 bits, see alignment 6.5e-18

Best Hits

Swiss-Prot: 99% identical to DHAR_ECOLI: PTS-dependent dihydroxyacetone kinase operon regulatory protein (dhaR) from Escherichia coli (strain K12)

KEGG orthology group: K05880, transcriptional activator for dhaKLM operon (inferred from 100% identity to ect:ECIAI39_1537)

Predicted SEED Role

"Phosphoenolpyruvate-dihydroxyacetone phosphotransferase operon regulatory protein DhaR" in subsystem Dihydroxyacetone kinases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (639 amino acids)

>HEPCGN_24870 dihydroxyacetone kinase operon transcriptional regulator DhaR (Escherichia coli ECOR38)
MSGAFNNDGRDISPLIATSWERCNKLMKRETWNVPHQAQGVTFASIYRRKKAMLTLGQAA
LEDAWEYMAPRACALLILDETACILSRNGDPQTLQQLSTLGFNDGTYCAEGIIGTCALSL
AAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGTIALACPVEQTTAADLPLTLA
IAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVISWDEQGNLQFINAQAARVLRLDA
TASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFESQHQFIDAVITLKPIIETQGTSFI
LLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQTRRLIHFGRQAARSSFPVLLCGEEGVG
KALLSQAIHNESERAAGPYIAVNCELYGDAALAEEFIGGDRTDNENGRLSRLELAHGGTL
FLEKIEYLAVELQSALLQVIKQGVITRLDARRLIPIDVKVIATTTADLAMLVEQNRFSRQ
LYYALHAFEITIPPLRMRRGSIPALVNNKLRSLEKRFSTRLKIDDDALARLVSCAWPGND
FELYSVIENLALSSDNGRIRVSDLPEHLFTEQATDDVSATRLSTSLSFAEVEKEAIINAA
QVTGGRIQEMSALLGIGRTTLWRKMKQHGIDAGQFKRRG