Protein Info for HEPCGN_21475 in Escherichia coli ECOR38

Annotation: RNA-guided endonuclease TnpB family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 268 PF01385: OrfB_IS605" amino acids 28 to 143 (116 residues), 118.9 bits, see alignment E=1.6e-38 TIGR01766: transposase, IS605 OrfB family" amino acids 110 to 197 (88 residues), 39 bits, see alignment E=4.5e-14 PF07282: OrfB_Zn_ribbon" amino acids 170 to 238 (69 residues), 88.2 bits, see alignment E=2.6e-29

Best Hits

KEGG orthology group: K07496, putative transposase (inferred from 97% identity to sfx:S2072)

Predicted SEED Role

"putative virulence protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (268 amino acids)

>HEPCGN_21475 RNA-guided endonuclease TnpB family protein (Escherichia coli ECOR38)
MRYRNSRQVTGVVKNVTVSQSCGKWYISIQTESEVSTPVHPSASMVGLDAGVAKLATLSD
GTVFEPVNSFQKKQKTLARLQRQLSRKVEFSNNWQKQKRKIQRLHSRIANIRRDYLHKVT
TTVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSVLDQGWYEMRRQLEYK
QLWRGGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQICGYTANADVNGARNILAAG
HAVLACGEMVQSGRPLKQEPTEMIQATA