Protein Info for HEPCGN_21300 in Escherichia coli ECOR38

Name: pbp4b
Annotation: penicillin binding protein PBP4B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 433 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details PF00144: Beta-lactamase" amino acids 44 to 412 (369 residues), 238.3 bits, see alignment E=6.6e-75

Best Hits

Swiss-Prot: 99% identical to YFEW_ECOLI: Putative D-alanyl-D-alanine carboxypeptidase (yfeW) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 99% identity to eco:b2430)

MetaCyc: 99% identical to penicillin binding protein 4B (Escherichia coli K-12 substr. MG1655)
Serine-type D-Ala-D-Ala carboxypeptidase. [EC: 3.4.16.4]

Predicted SEED Role

"Beta-lactamase class C and other penicillin binding proteins" in subsystem Beta-lactamase

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.16.4

Use Curated BLAST to search for 3.4.16.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (433 amino acids)

>HEPCGN_21300 penicillin binding protein PBP4B (Escherichia coli ECOR38)
MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMDRWISQQVDAGYPGVNL
LIIKDNQIVYRKAWGAAKKYDGSTLMAQPVKATTGTLYDLASNTKMYATNFALQKLMSEG
KLHPDDRIAKYIPGFADNPNDTIKGKNTLRISDLLHHSGGFPADPQYPNKAVAGALYSQD
KGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESVTGQPLDRYVEESIYRPLGLTHT
VFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTSTLWGQVHDEKAFYSMGGVSGHAGL
FSNTGDIAVLMQTMLNGGGYGDVQLFNAETVKMFTTSSKEDATFGLGWRVNGNATMTPTF
GTLASPQTYGHTGWTGTVTVIDPVNHMAIVMLSNKPHSPVADPQKNPNMFESGQLPIATY
GWVVDQVYAALKQ