Protein Info for HEPCGN_19880 in Escherichia coli ECOR38

Name: srlE
Annotation: PTS glucitol/sorbitol transporter subunit IIB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 319 transmembrane" amino acids 147 to 165 (19 residues), see Phobius details amino acids 177 to 197 (21 residues), see Phobius details amino acids 207 to 227 (21 residues), see Phobius details amino acids 232 to 251 (20 residues), see Phobius details amino acids 257 to 280 (24 residues), see Phobius details amino acids 296 to 318 (23 residues), see Phobius details TIGR00825: PTS system, glucitol/sorbitol-specific, IIBC component" amino acids 3 to 319 (317 residues), 629.5 bits, see alignment E=5.5e-194 PF03612: EIIBC-GUT_N" amino acids 4 to 171 (168 residues), 219.4 bits, see alignment E=3.4e-69 PF07663: EIIBC-GUT_C" amino acids 226 to 318 (93 residues), 145.6 bits, see alignment E=4.2e-47

Best Hits

Swiss-Prot: 99% identical to PTHB_ECOLI: PTS system glucitol/sorbitol-specific EIIB component (srlE) from Escherichia coli (strain K12)

KEGG orthology group: K02782, PTS system, glucitol/sorbitol-specific IIB component [EC: 2.7.1.69] K02783, PTS system, glucitol/sorbitol-specific IIC component (inferred from 99% identity to eco:b2703)

MetaCyc: 99% identical to sorbitol-specific PTS enzyme IIBC1 component (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-169 [EC: 2.7.1.198]; TRANS-RXN-156 [EC: 2.7.1.198, 2.7.1.197]

Predicted SEED Role

"PTS system, glucitol/sorbitol-specific IIB component and second of two IIC components (EC 2.7.1.69)" in subsystem D-Sorbitol(D-Glucitol) and L-Sorbose Utilization (EC 2.7.1.69)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69

Use Curated BLAST to search for 2.7.1.197 or 2.7.1.198 or 2.7.1.69

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (319 amino acids)

>HEPCGN_19880 PTS glucitol/sorbitol transporter subunit IIB (Escherichia coli ECOR38)
MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQLTGWQAIDGFKEGEPA
EEEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQYIVEDIYVSGVKEENITVV
GDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVAVLFQSGRDTIDTVLKTILPF
MAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLALICSFPLLSPFLGPGAVIAQVI
GVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPVGLSLAEARQDTVRVGVPSVLVSRF
LTGAPTVLIAWFVSGFIYQ