Protein Info for HEPCGN_18810 in Escherichia coli ECOR38

Name: gcvT
Annotation: glycine cleavage system aminomethyltransferase GcvT

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to GCST_ECO7I: Aminomethyltransferase (gcvT) from Escherichia coli O7:K1 (strain IAI39 / ExPEC)

KEGG orthology group: K00605, aminomethyltransferase [EC: 2.1.2.10] (inferred from 100% identity to eco:b2905)

MetaCyc: 100% identical to aminomethyltransferase (Escherichia coli K-12 substr. MG1655)
Aminomethyltransferase. [EC: 1.4.1.27, 2.1.2.10]; 1.4.1.27 [EC: 1.4.1.27, 2.1.2.10]

Predicted SEED Role

"Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10)" in subsystem Glycine and Serine Utilization or Glycine cleavage system or Photorespiration (oxidative C2 cycle) (EC 2.1.2.10)

MetaCyc Pathways

folate transformations III (E. coli) (9/9 steps found)
folate transformations II (plants) (10/11 steps found)
glycine biosynthesis II (3/3 steps found)
glycine cleavage (3/3 steps found)
glycine degradation (3/3 steps found)
folate transformations I (9/13 steps found)
reductive glycine pathway of autotrophic CO₂ fixation (6/9 steps found)
photorespiration II (6/10 steps found)
photorespiration I (5/9 steps found)
photorespiration III (5/9 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.1.27 or 2.1.2.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (364 amino acids)

>HEPCGN_18810 glycine cleavage system aminomethyltransferase GcvT (Escherichia coli ECOR38)
MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDAGMFDVSHMTIVDLRGS
RTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVYYFTEDFFRLVVNSATREK
DLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLFNDAQRQAVEGMKPFFGVQAG
DLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKPCGLGARDTLRLEAGMNLYSQEM
DETISPLAANMGWTIAWEPADRDFIGREALEAQREHGTEKLVGLVMTEKGVLRNELPVRF
TDAQGNQHEGIITSGTFSPTLGYSIALARVPEGIGETAIVQIRNREMPVKVTKPVFVRNG
KAVA