Protein Info for HEPCGN_18285 in Escherichia coli ECOR38

Name: iucA
Annotation: aerobactin synthase IucA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 574 transmembrane" amino acids 480 to 501 (22 residues), see Phobius details PF04183: IucA_IucC" amino acids 136 to 364 (229 residues), 155.9 bits, see alignment E=1.4e-49 PF06276: FhuF" amino acids 398 to 554 (157 residues), 118.1 bits, see alignment E=5.7e-38

Best Hits

Swiss-Prot: 93% identical to IUCA_ECOLX: N(2)-citryl-N(6)-acetyl-N(6)-hydroxylysine synthase (iucA) from Escherichia coli

KEGG orthology group: K03894, aerobactin synthetase subunit alpha [EC: 6.3.2.27] (inferred from 100% identity to ect:ECIAI39_3423)

MetaCyc: 93% identical to citrate:N6-acetyl-N6-hydroxy-L-lysine ligase (Escherichia coli K-12)
RXN-13054 [EC: 6.3.2.38]

Predicted SEED Role

"Citrate:6-N-acetyl-6-N-hydroxy-L-lysine ligase, alpha subunit (EC 6.3.2.27), aerobactin biosynthesis protein IucA @ Siderophore synthetase superfamily, group A @ Siderophore synthetase large component, acetyltransferase" (EC 6.3.2.27)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.2.27

Use Curated BLAST to search for 6.3.2.27 or 6.3.2.38

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (574 amino acids)

>HEPCGN_18285 aerobactin synthase IucA (Escherichia coli ECOR38)
MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDEQKSLHFRVAYFSPTQH
HRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLCETFHQRVLESHVHTQQAI
DARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFNRREAERYLPDMAPHFPLRWF
SVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDNQWLFPLHPWQGEYLLQQGWCQA
LVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIKFSLSVRLTNSIRTLSVKEVKRGMR
LARLAQTDGWQMLQVRFPTFRVMQEDGWAGLRDLNGNIMQESLFALRENLLVDQPKSQTN
VLVSLTQAAPDGGDSLLVSAVKRLSDRLGITVQQAAHAWVDAYCQQVLKPLFTAEADYGL
VLLAHQQNILVQMLGDLPVGFIYRDCQGSAFMPHATDWLDSIGEAQAENIFTHEQLLRYF
PYYLLVNSTFAVTAALGAAGLDSESNLMARVRASLAEVRDQVTHKTCLNYVLESPYWNVK
GNFFCYLNDHNENTIVDPSVIYFDFANPLQAQEV