Protein Info for HEPCGN_18125 in Escherichia coli ECOR38

Name: neuC
Annotation: UDP-N-acetylglucosamine 2-epimerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02350: Epimerase_2
  • amino acids 2 to 345 (344 residues), 243 bits, E=2.2e-76
• TIGR03568: UDP-N-acetyl-D-glucosamine 2-epimerase, UDP-hydrolysing
  • amino acids 2 to 344 (343 residues), 405.4 bits, E=1e-125

Best Hits

Swiss-Prot: 100% identical to NEUC_ECOLX: Polysialic acid biosynthesis protein P7 (neuC) from Escherichia coli

KEGG orthology group: K01791, UDP-N-acetylglucosamine 2-epimerase [EC: 5.1.3.14] (inferred from 100% identity to ecm:EcSMS35_3230)

MetaCyc: 100% identical to UDP-N-acetylglucosamine 2-epimerase (Escherichia coli K1)
RXN-9987 [EC: 3.2.1.183]

Predicted SEED Role

"UDP-N-acetylglucosamine 2-epimerase (EC 5.1.3.14)" in subsystem CMP-N-acetylneuraminate Biosynthesis or Sialic Acid Metabolism (EC 5.1.3.14)

MetaCyc Pathways

• CMP-N-acetylneuraminate biosynthesis II (bacteria) (3/3 steps found)
• UDP-N-acetyl-α-D-mannosamine biosynthesis (1/1 steps found)
• CMP-N-acetylneuraminate biosynthesis I (eukaryotes) (3/5 steps found)
• superpathway of CMP-sialic acids biosynthesis (5/15 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (7/24 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 5.1.3.14

Use Curated BLAST to search for 3.2.1.183 or 5.1.3.14

Protein Sequence (369 amino acids)

>HEPCGN_18125 UDP-N-acetylglucosamine 2-epimerase (Escherichia coli ECOR38)
MLRETPEIQLDLAVTGMHCDNAYGNTIHIIEQDNFNIIKVVDININTTSHTHILHSMSVC
LNSFGDFFSNNTYDAVMVLGDRYEIFSVAIAASMHNIPLIHIHGGEKTLANYDEFIRHSI
TKMSKLHLTSTEEYKKRVIQLGEKPGSVFNIGSLGAENALSLHLPNKQELELKYGSLLKR
YFVVVFHPETLSTQSVNDQIDELLSAISFFKNTHDFIFIGSNADTGSDIIQRKVKYFCKE
YKFRYLISIRSEDYLAMIKYSCGLIGNSSSGLIEVPSLKVATINIGDRQKGRVRGASVID
VPVEKNAIVRGINISQDEKFISVVQSSSNPYFKENALINAVRIIKDFIKSKNKDYKDFYD
IPECTTSYD