Protein Info for HEPCGN_14460 in Escherichia coli ECOR38

Name: mutM
Annotation: bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 269 TIGR00577: DNA-formamidopyrimidine glycosylase" amino acids 1 to 268 (268 residues), 359.3 bits, see alignment E=6.5e-112 PF01149: Fapy_DNA_glyco" amino acids 1 to 114 (114 residues), 123.1 bits, see alignment E=1.5e-39 PF06831: H2TH" amino acids 129 to 219 (91 residues), 114.7 bits, see alignment E=2.3e-37 PF06827: zf-FPG_IleRS" amino acids 241 to 269 (29 residues), 35.8 bits, see alignment (E = 8.7e-13)

Best Hits

Swiss-Prot: 100% identical to FPG_ECO7I: Formamidopyrimidine-DNA glycosylase (mutM) from Escherichia coli O7:K1 (strain IAI39 / ExPEC)

KEGG orthology group: K10563, formamidopyrimidine-DNA glycosylase [EC: 3.2.2.23 4.2.99.18] (inferred from 99% identity to ecx:EcHS_A3844)

MetaCyc: 99% identical to DNA-formamidopyrimidine glycosylase (Escherichia coli K-12 substr. MG1655)
DNA-formamidopyrimidine glycosylase. [EC: 3.2.2.23]; 3.2.2.23 [EC: 3.2.2.23]; 3.2.2.23 [EC: 3.2.2.23]; RXN-21279 [EC: 3.2.2.23]

Predicted SEED Role

"Formamidopyrimidine-DNA glycosylase (EC 3.2.2.23)" in subsystem DNA Repair Base Excision (EC 3.2.2.23)

Isozymes

Compare fitness of predicted isozymes for: 4.2.99.18

Use Curated BLAST to search for 3.2.2.23 or 4.2.99.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (269 amino acids)

>HEPCGN_14460 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase (Escherichia coli ECOR38)
MPELPEVETSRRGIEPYLVGATILHAVVRNGRLRWPVSEEIYRLSDQPVLSVQRRAKYLL
LELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKVLRYTDPRRFGAWLWTKEL
EGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMDNKLVVGVGNIYASESLFAAG
IHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLKDFLQSDGKPGYFAQELQVYGRK
GEPCRVCGTPIVATKHAQRATFYCRQCQK