Protein Info for HEPCGN_10510 in Escherichia coli ECOR38

Name: nnr
Annotation: bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 510 TIGR00197: YjeF family N-terminal domain" amino acids 12 to 215 (204 residues), 239.3 bits, see alignment E=3.3e-75 PF03853: YjeF_N" amino acids 35 to 194 (160 residues), 151.8 bits, see alignment E=1.9e-48 TIGR00196: YjeF family C-terminal domain" amino acids 226 to 494 (269 residues), 329.6 bits, see alignment E=1.5e-102 PF01256: Carb_kinase" amino acids 253 to 491 (239 residues), 285.2 bits, see alignment E=4.2e-89

Best Hits

Swiss-Prot: 98% identical to NNR_ECOLI: Bifunctional NAD(P)H-hydrate repair enzyme Nnr (nnr) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to ect:ECIAI39_4631)

MetaCyc: 98% identical to NAD(P)HX epimerase / NAD(P)HX dehydratase (Escherichia coli K-12 substr. MG1655)
RXN-12752 [EC: 5.1.99.6]; 5.1.99.6 [EC: 5.1.99.6]; RXN-13141 [EC: 5.1.99.6, 4.2.1.136]; 4.2.1.136 [EC: 5.1.99.6, 4.2.1.136]

Predicted SEED Role

"NAD(P)HX epimerase / NAD(P)HX dehydratase"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.136 or 5.1.99.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (510 amino acids)

>HEPCGN_10510 bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase (Escherichia coli ECOR38)
MKKNPVSIPHTVWHADDIRRGEGEAADALGLTLYELMLRAGEAAFQVCRSAYPDARHWQV
LCGHGNNGGDGYVVARLAKAIGIDVTLLAQESDKPLPEEAALAREAWLNAGGEIHASNIV
WPESVDLIVDALLGTGLQQAPRESISQLIDHANTHPAPIVAVDIPSGLLAETGATPGAVI
NADHTITFIALKPGLLTGKARDVTGQLHFDSLGLDSWLAGQETKIQRFSAEQLSHWLKPR
RPTSHKGDHGRLVIIGGDHGTAGAIRMTGEAALRAGAGLVRVLTRSENIAPLLTARPELM
VHELTMDSLTESLEWADVVVIGPGLGQQEWGKKALQKVENFRKPMLWDADALNLLAINPD
KRHNRVITPHPGEAARLLGCSVAEIESDRLHCAQRLVQRYGGVAVLKGAGTVVAAHPDAL
GIIDVGNAGMASGGMGDVLSGIIGALLGQKMSPYDAACAGCVAHGAAADVLAARFGTRGM
LATDLFSTLQRIVNPEVTDKNHDESSNSAP