Protein Info for HEPCGN_09725 in Escherichia coli ECOR38

Name: istA
Annotation: IS21 family transposase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 220 PF01695: IstB_IS21" amino acids 1 to 211 (211 residues), 338.2 bits, see alignment E=3.9e-105 PF00004: AAA" amino acids 66 to 138 (73 residues), 24.3 bits, see alignment E=5.6e-09 PF00308: Bac_DnaA" amino acids 66 to 163 (98 residues), 30.2 bits, see alignment E=6.3e-11

Best Hits

Swiss-Prot: 50% identical to ISTB_PSEAI: Insertion sequence IS21 putative ATP-binding protein (istB) from Pseudomonas aeruginosa

KEGG orthology group: None (inferred from 100% identity to ecp:ECP_2996)

Predicted SEED Role

"Mobile element protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (220 amino acids)

>HEPCGN_09725 IS21 family transposase (Escherichia coli ECOR38)
MDFLEHLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFI
ERNENIVLLGPSGVGKTHLAIAMGYEAVRAGIKVRFTTAADLLLQLSTAQRQGRYKTTLQ
RGVMAPRLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAA
LTSAMLDRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE