Protein Info for HEPCGN_08465 in Escherichia coli ECOR38

Annotation: DUF3300 domain-containing protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 585 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details PF11737: DUF3300" amino acids 74 to 333 (260 residues), 262 bits, see alignment E=2.2e-82

Best Hits

Swiss-Prot: 90% identical to YACH_ECOLI: Uncharacterized protein YacH (yacH) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 99% identity to ecm:EcSMS35_0127)

Predicted SEED Role

"FIG01200701: possible membrane protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (585 amino acids)

>HEPCGN_08465 DUF3300 domain-containing protein (Escherichia coli ECOR38)
MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVESQSTQQTAPDISAVTLPATVS
APPVTPAVVKSTFSTAQIDQWVAPVALYPDSLLSQVLMASTYPANVAQAVQWSHDNPLKQ
GDAAIQAVSGQPWDASVKSLVAFPQLMALMGENPQWVQNLGDAFLAQPQDVMDSVQRLRQ
LAQQTGSLKSSTEQKIITTTKKVVPVNQPANAPATQSNTVSTSSPVVAEPAPTVITIEPA
NPDVVYIPNYNPNVVYGSWANTAYPPVYLPPPAGEPFVDSFVRGFGYSMGVATTYALFSS
IDWDDDDHDHHHHDDDDYHHHDGGHRDGNDWQHNGDNINIDVNNFNRITGEHLTDKNMAW
RHNPNYRDGVPYHDQDMAKRFHQTDVNGGMSATQLPALSRDSQRQAAASQFQQRTHAAPV
ITRDTQRQAAAQRFNEAENYGSYDDFRDFSRRQPLTQQQKDAARQRYQSASPEQRQAVRE
KMQTNPQIQQRREAARERIQSATPEQRQVFKEKVQQRPLNQQQRDNARQRIQSASPEQRQ
VFREKVQESRPQRLNDSNHTVRLNNEQRSAVRERLSERGARRLER