Protein Info for HEPCGN_06995 in Escherichia coli ECOR38

Name: cyoE
Annotation: heme o synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 296 transmembrane" amino acids 12 to 30 (19 residues), see Phobius details amino acids 38 to 60 (23 residues), see Phobius details amino acids 80 to 102 (23 residues), see Phobius details amino acids 108 to 127 (20 residues), see Phobius details amino acids 134 to 156 (23 residues), see Phobius details amino acids 162 to 183 (22 residues), see Phobius details amino acids 209 to 227 (19 residues), see Phobius details amino acids 233 to 252 (20 residues), see Phobius details amino acids 264 to 285 (22 residues), see Phobius details TIGR01473: protoheme IX farnesyltransferase" amino acids 4 to 282 (279 residues), 305.2 bits, see alignment E=2.9e-95 PF01040: UbiA" amino acids 19 to 265 (247 residues), 212.3 bits, see alignment E=3.5e-67

Best Hits

Swiss-Prot: 100% identical to CYOE_SHIBS: Protoheme IX farnesyltransferase (cyoE) from Shigella boydii serotype 4 (strain Sb227)

KEGG orthology group: K02301, protoheme IX farnesyltransferase [EC: 2.5.1.-] (inferred from 100% identity to eco:b0428)

MetaCyc: 100% identical to heme O synthase (Escherichia coli K-12 substr. MG1655)
HEMEOSYN-RXN [EC: 2.5.1.141]

Predicted SEED Role

No annotation

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.-

Use Curated BLAST to search for 2.5.1.- or 2.5.1.141

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (296 amino acids)

>HEPCGN_06995 heme o synthase (Escherichia coli ECOR38)
MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLVGVSLVVASGCVFNNYI
DRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLWFGANPLACWLGVMGFVVY
VGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDSGAAILLAIFSLWQMPHSYAI
AIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAVATLMLSLGGYAGYKYLVVAAAV
SVWWLGMALRGYKVADDRIWARKLFGFSIIAITALSVMMSVDFMVPDSHTLLAAVW