Protein Info for HEPCGN_05200 in Escherichia coli ECOR38

Name: topB
Annotation: DNA topoisomerase III

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 651 TIGR01056: DNA topoisomerase III" amino acids 1 to 649 (649 residues), 1017.8 bits, see alignment E=0 PF01751: Toprim" amino acids 3 to 135 (133 residues), 56.7 bits, see alignment E=2.3e-19 PF01131: Topoisom_bac" amino acids 158 to 593 (436 residues), 365.7 bits, see alignment E=3.5e-113

Best Hits

Swiss-Prot: 96% identical to TOP3_ECOLI: DNA topoisomerase 3 (topB) from Escherichia coli (strain K12)

KEGG orthology group: K03169, DNA topoisomerase III [EC: 5.99.1.2] (inferred from 100% identity to ect:ECIAI39_1293)

MetaCyc: 96% identical to DNA topoisomerase III (Escherichia coli K-12 substr. MG1655)
5.99.1.2-RXN [EC: 5.6.2.1]

Predicted SEED Role

"DNA topoisomerase III (EC 5.99.1.2)" in subsystem DNA topoisomerases, Type I, ATP-independent (EC 5.99.1.2)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.2

Use Curated BLAST to search for 5.6.2.1 or 5.99.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (651 amino acids)

>HEPCGN_05200 DNA topoisomerase III (Escherichia coli ECOR38)
MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGHLLEQAQPDAYDSRYAR
WNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAGDPDREGQLLVDEVLDYLQ
LAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCVSALARARADWLYGINMTRAY
TILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVAKDFFEVKAHIVTPADERFTAIW
QPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIVTSYNDKRESESAPLPFSLSALQIE
AAKRFGLSAQNVLDICQKLYETHKLITYPRSDCRYLPEEHFAGRHAVMNAISVHAPGLLP
QPVVDPDIRNRCWDDKKVDAHHAIIPTARSSAINLTENEAKVYNLIARQYLMQFCPDAVF
RKCVIELDIAKGKFVAKARFLAEAGWRTLLGSKERDEENDGMPLPVVAKGDELLCEKGEV
VERQTQPPRHFTDATLLSAMTGIARFVQDKDLKKILRATDGLGTEATRAGIIELLFKRGF
LTKKGRYIHSTDAGKALFHSLPEMATRPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLY
QLIDQAKRTSVRQFRGIMAPGGGEGKKKDSPRKRAPKKSPPSEEAGNGVII