Protein Info for HEPCGN_04630 in Escherichia coli ECOR38

Name: cmoB
Annotation: tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 323 PF08003: Methyltransf_9" amino acids 7 to 322 (316 residues), 576.5 bits, see alignment E=3e-177 TIGR00452: tRNA (mo5U34)-methyltransferase" amino acids 8 to 321 (314 residues), 492 bits, see alignment E=2.8e-152 PF13489: Methyltransf_23" amino acids 112 to 269 (158 residues), 37.4 bits, see alignment E=5.6e-13 PF13847: Methyltransf_31" amino acids 123 to 228 (106 residues), 29.4 bits, see alignment E=1.4e-10 PF13649: Methyltransf_25" amino acids 126 to 204 (79 residues), 29.4 bits, see alignment E=2.7e-10 PF08241: Methyltransf_11" amino acids 127 to 223 (97 residues), 25.4 bits, see alignment E=4.4e-09

Best Hits

Swiss-Prot: 100% identical to CMOB_ECOLC: tRNA U34 carboxymethyltransferase (cmoB) from Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks)

KEGG orthology group: K15257, tRNA (mo5U34)-methyltransferase [EC: 2.1.1.-] (inferred from 100% identity to eco:b1871)

MetaCyc: 100% identical to tRNA U34 carboxymethyltransferase (Escherichia coli K-12 substr. MG1655)
RXN0-7067

Predicted SEED Role

"tRNA (5-methoxyuridine) 34 synthase"

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (323 amino acids)

>HEPCGN_04630 tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB (Escherichia coli ECOR38)
MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSNAVEFLPEIKPYRLDLL
HSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNIDTEWRSDWKWDRVLPHLSD
LTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQFEAVRKLLGNDQRAHLLPLG
IEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVNEGELVLETLVIDGDENTVLVPG
DRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVSVTTTEEQRRTEWMVTESLADFLDP
HDPSKTVEGYPAPKRAVLIARKP