Protein Info for HEPCGN_02755 in Escherichia coli ECOR38

Name: thiM
Annotation: hydroxyethylthiazole kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00694: hydroxyethylthiazole kinase
  • amino acids 15 to 261 (247 residues), 331 bits, E=2.5e-103
• PF02110: HK
  • amino acids 15 to 259 (245 residues), 354.6 bits, E=3.9e-110
• PF01256: Carb_kinase
  • amino acids 42 to 238 (197 residues), 27.3 bits, E=3.8e-10
• PF08543: Phos_pyr_kin
  • amino acids 79 to 240 (162 residues), 28.2 bits, E=1.8e-10

Best Hits

Swiss-Prot: 100% identical to THIM_ECO7I: Hydroxyethylthiazole kinase (thiM) from Escherichia coli O7:K1 (strain IAI39 / ExPEC)

KEGG orthology group: K00878, hydroxyethylthiazole kinase [EC: 2.7.1.50] (inferred from 98% identity to ecp:ECP_2142)

MetaCyc: 95% identical to hydroxyethylthiazole kinase (Escherichia coli K-12 substr. MG1655)
Hydroxyethylthiazole kinase. [EC: 2.7.1.50]; 2.7.1.50 [EC: 2.7.1.50]; Choline kinase. [EC: 2.7.1.50, 2.7.1.32]

Predicted SEED Role

"Hydroxyethylthiazole kinase (EC 2.7.1.50)" in subsystem Thiamin biosynthesis (EC 2.7.1.50)

MetaCyc Pathways

• thiamine diphosphate salvage II (5/5 steps found)
• prenylated FMNH₂ biosynthesis (3/3 steps found)
• thiamine diphosphate salvage V (3/3 steps found)
• thiamine diphosphate salvage IV (yeast) (4/7 steps found)
• thiamine diphosphate formation from pyrithiamine and oxythiamine (yeast) (4/8 steps found)
• superpathway of phospholipid biosynthesis II (plants) (10/28 steps found)
• plasmalogen biosynthesis I (aerobic) (1/16 steps found)

KEGG Metabolic Maps

• Glycerophospholipid metabolism
• Thiamine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.32 or 2.7.1.50

Protein Sequence (262 amino acids)

>HEPCGN_02755 hydroxyethylthiazole kinase (Escherichia coli ECOR38)
MQVDLLSSAQSAHALHLFHKHSPLVHCMTNDVVQTFTANTLLALGASPAMVIETEEASQF
AAIASALLINVGTLTQPRAQAMSAAVEQATRSQTPWTLDPVAVGALDYRRRFCVELLSHK
PTAIRGNASEIMALAGVANGGRGVDTTDAAANAIPAAQTLARETGAIVVVTGEVDYVTDG
HRIVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTLENIASACHWMKQAGERAVARSE
GPGSFVPHFLDALWQLTQEVQA