Protein Info for HEPCGN_02150 in Escherichia coli ECOR38

Name: amiD
Annotation: N-acetylmuramoyl-L-alanine amidase AmiD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01510: Amidase_2
  • amino acids 42 to 179 (138 residues), 105.2 bits, E=1.7e-34

Best Hits

Swiss-Prot: 99% identical to AMID_ECOLI: N-acetylmuramoyl-L-alanine amidase AmiD (amiD) from Escherichia coli (strain K12)

KEGG orthology group: K11066, N-acetylmuramoyl-L-alanine amidase [EC: 3.5.1.28] (inferred from 99% identity to eco:b0867)

MetaCyc: 99% identical to N-acetylmuramoyl-L-alanine amidase D (Escherichia coli K-12 substr. MG1655)
N-acetylmuramoyl-L-alanine amidase. [EC: 3.5.1.28]

Predicted SEED Role

"N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)" (EC 3.5.1.28)

MetaCyc Pathways

• peptidoglycan recycling I (14/14 steps found)
• peptidoglycan recycling II (7/10 steps found)

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.28

Use Curated BLAST to search for 3.5.1.28

Protein Sequence (276 amino acids)

>HEPCGN_02150 N-acetylmuramoyl-L-alanine amidase AmiD (Escherichia coli ECOR38)
MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRIKVLVIHYTADDFDSSL
ATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGISAWRGATRLNDTSIGIEL
ENRGWQKSAGVKYFAPFEQAQIQALIPLAKDIIARYHIKPENVVAHADIAPQRKDDPGPL
FPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASLLELLARYGYDVKPDMTPREQRR
VIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD