Protein Info for HEPCGN_00945 in Escherichia coli ECOR38

Name: leuS
Annotation: leucine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 860 transmembrane" amino acids 520 to 541 (22 residues), see Phobius details TIGR00396: leucine--tRNA ligase" amino acids 5 to 859 (855 residues), 1315.7 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 15 to 225 (211 residues), 108.8 bits, see alignment E=5.6e-35 amino acids 417 to 572 (156 residues), 32 bits, see alignment E=1.2e-11 amino acids 618 to 652 (35 residues), 27.6 bits, see alignment (E = 2.5e-10) PF09334: tRNA-synt_1g" amino acids 39 to 182 (144 residues), 81.9 bits, see alignment E=8.4e-27 amino acids 604 to 659 (56 residues), 21.6 bits, see alignment 1.7e-08 PF13603: tRNA-synt_1_2" amino acids 221 to 403 (183 residues), 268.9 bits, see alignment E=3.9e-84 PF08264: Anticodon_1" amino acids 699 to 822 (124 residues), 72.5 bits, see alignment E=7.3e-24

Best Hits

Swiss-Prot: 100% identical to SYL_ECO7I: Leucine--tRNA ligase (leuS) from Escherichia coli O7:K1 (strain IAI39 / ExPEC)

KEGG orthology group: K01869, leucyl-tRNA synthetase [EC: 6.1.1.4] (inferred from 100% identity to ebd:ECBD_3009)

MetaCyc: 99% identical to leucine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Leucine--tRNA ligase. [EC: 6.1.1.4]; 6.1.1.4 [EC: 6.1.1.4]; 3.1.1.- [EC: 6.1.1.4]

Predicted SEED Role

"Leucyl-tRNA synthetase (EC 6.1.1.4)" (EC 6.1.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (860 amino acids)

>HEPCGN_00945 leucine--tRNA ligase (Escherichia coli ECOR38)
MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYPSGRLHMGHVRNYTIGD
VIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTYDNIAYMKNQLKMLGFGYD
WSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCPNDQTVLANEQVIDGCCWRCD
TKVERKEIPQWFIKITAYADELLNNLDKLDHWPDTVKTMQRNWIGRSEGVEITFNVNDYD
NTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNPELAAFIDECRNTKVAEAEMATMEK
KGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGAVMAVPGHDQRDYEFASKYGLNIKPVI
LAADGSEPDLSQQALTEKGVLFNSGEFNGLDHEAAFNAIADKLTAMGVGERKVNYRLRDW
GVSRQRYWGAPIPMVTLEDGTVMPTPDDQLPVILPEDVVMDGITSPIKADPEWAKTTVNG
MPALRETDTFDTFMESSWYYARYTCPEYKEGMLDSKAANYWLPVDIYIGGIEHAIMHLLY
FRFFHKLMRDAGMVNSDEPAKQLLCQGMVLADAFYYVGENGERNWVSPVDAIVERDEKGR
IVKAKDAAGHELVYTGMSKMSKSKNNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQE
SGVEGANRFLKRVWKLVYEHTAKGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQ
TFNTAIAAIMELMNKLAKAPTDGEQDRALMQEALLAVIRMLNPFTPHICFTLWQELKGEG
DIDNAPWPVADEKAMVEDSTLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLD
GVTVRKVIYVPGKLLNLVVG