Protein Info for GFF999 in Variovorax sp. SCN45

Annotation: GTP-binding protein EngA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 447 TIGR03594: ribosome-associated GTPase EngA" amino acids 3 to 436 (434 residues), 568.8 bits, see alignment E=1.3e-174 TIGR00231: small GTP-binding protein domain" amino acids 4 to 158 (155 residues), 73.2 bits, see alignment E=3.2e-24 amino acids 179 to 333 (155 residues), 88.1 bits, see alignment E=8.1e-29 PF01926: MMR_HSR1" amino acids 5 to 120 (116 residues), 100.2 bits, see alignment E=2.7e-32 amino acids 183 to 300 (118 residues), 88.7 bits, see alignment E=1.1e-28 PF02421: FeoB_N" amino acids 5 to 156 (152 residues), 53.1 bits, see alignment E=9.4e-18 amino acids 182 to 320 (139 residues), 42 bits, see alignment E=2.4e-14 PF00009: GTP_EFTU" amino acids 213 to 348 (136 residues), 33.9 bits, see alignment E=8.7e-12 PF14714: KH_dom-like" amino acids 356 to 436 (81 residues), 87.5 bits, see alignment E=2e-28

Best Hits

Swiss-Prot: 96% identical to DER_VARPS: GTPase Der (der) from Variovorax paradoxus (strain S110)

KEGG orthology group: K03977, GTP-binding protein (inferred from 97% identity to vpe:Varpa_3699)

Predicted SEED Role

"GTP-binding protein EngA" in subsystem Universal GTPases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (447 amino acids)

>GFF999 GTP-binding protein EngA (Variovorax sp. SCN45)
MKPVVALVGRPNVGKSTLFNRLTQTRDAIVADFAGLTRDRHYGNGRLGKHEFIVIDTGGF
EPDAGSGIYKEMAKQTRQAVAEADVVIFVVDAREGLSAQDHDIANELRRLGKPCVLAANK
AEGMHDGTKLVDFYELGFGDVHGVSAAHGQGMRDLVELALEPLNLPDPDDESEEDEANKP
IKLAVAGRPNVGKSTLINTWLGEERLVAFDMPGTTRDAISVPFERNGQRFELIDTAGLRR
KGRVFEAIEKFSVVKTLQAIESANVVLLLLDATQGVTDQDAHIAGYILESGRAVVIAINK
WDAVDSYQREQIQRQIETRLPFLKFAALHFISAIKRQGLGPVWQAIAQAHKSATRKMSTP
VLTRLLLEAVQFQAPKRGGMYRPKLRYAHQGGMNPPVIVIHGNSLEHVTDAYKRFLEGRF
RKEFDLVGTPLRIEFKSSHNPFADKDD