Protein Info for GFF996 in Variovorax sp. SCN45

Annotation: Histidyl-tRNA synthetase (EC 6.1.1.21)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 436 TIGR00442: histidine--tRNA ligase" amino acids 7 to 413 (407 residues), 482.5 bits, see alignment E=5.3e-149 PF13393: tRNA-synt_His" amino acids 10 to 316 (307 residues), 152.2 bits, see alignment E=3.1e-48 PF00587: tRNA-synt_2b" amino acids 81 to 322 (242 residues), 59.3 bits, see alignment E=8e-20 PF03129: HGTP_anticodon" amino acids 342 to 411 (70 residues), 26.7 bits, see alignment E=7.9e-10

Best Hits

Swiss-Prot: 70% identical to SYH_ACISJ: Histidine--tRNA ligase (hisS) from Acidovorax sp. (strain JS42)

KEGG orthology group: K01892, histidyl-tRNA synthetase [EC: 6.1.1.21] (inferred from 88% identity to vpe:Varpa_3702)

MetaCyc: 53% identical to histidine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Histidine--tRNA ligase. [EC: 6.1.1.21]

Predicted SEED Role

"Histidyl-tRNA synthetase (EC 6.1.1.21)" (EC 6.1.1.21)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (436 amino acids)

>GFF996 Histidyl-tRNA synthetase (EC 6.1.1.21) (Variovorax sp. SCN45)
MADKLNAVKGMNDILPPESARWEWLEATVRDLMGRFAYRNVRTPILERTALFVRGIGEVT
DIVEKEMYSFEDRADKHGKFEHLTMRPENTAGLVRAVIEHNMLYEGPKRLWYTGPMFRRE
NVQRGRFRQFHQIGAEALGFAGPDVDAELILLANALWKAIGLTDVRLELNSLGQPAERAL
HRTELIAHFEKHADKLDEDGKRRLHSNPLRILDTKNPAMKDVVESAPKLIDFLGAESLAH
FEGLQAILKANDIAFTINPRLVRGLDYYNLSVFEFVTDRLGSQGTVCAGGRYDDLIAQIG
GKPAPAVGWAMGVERVLDLLKEQGAEVPAPVPDAYAIVPDAASMPVALRTLQQLRESGVR
VQMHAATVDGLSGFKAQMKKADASGATYALIFGADELARGEVTLKALRDGSGAQTSRALA
EAPIWAATLQSPAPNT