Protein Info for GFF993 in Methylophilus sp. DMC18

Annotation: Peptide chain release factor 3

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 524 TIGR00503: peptide chain release factor 3" amino acids 1 to 522 (522 residues), 789.1 bits, see alignment E=1.7e-241 PF00009: GTP_EFTU" amino acids 10 to 272 (263 residues), 179.8 bits, see alignment E=1.1e-56 PF01926: MMR_HSR1" amino acids 14 to 141 (128 residues), 24.2 bits, see alignment E=7.6e-09 TIGR00231: small GTP-binding protein domain" amino acids 14 to 146 (133 residues), 70.6 bits, see alignment E=1.4e-23 PF22042: EF-G_D2" amino acids 290 to 376 (87 residues), 88.3 bits, see alignment E=7.2e-29 PF03144: GTP_EFTU_D2" amino acids 309 to 375 (67 residues), 35.9 bits, see alignment E=2.1e-12 PF16658: RF3_C" amino acids 382 to 508 (127 residues), 145.9 bits, see alignment E=1.6e-46

Best Hits

Swiss-Prot: 88% identical to RF3_METFK: Peptide chain release factor 3 (prfC) from Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875)

KEGG orthology group: K02837, peptide chain release factor 3 (inferred from 92% identity to mmb:Mmol_1410)

Predicted SEED Role

"Peptide chain release factor 3"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (524 amino acids)

>GFF993 Peptide chain release factor 3 (Methylophilus sp. DMC18)
MSSLQQEVERRRTFAIISHPDAGKTTLTEKLLWFGGAIQVAGEVRGRKAARHATSDWMEL
EKQRGISVTSSVMQFPYREHMVNLLDTPGHDDFSEDTYRTLTAVDSAVMVIDSVNGVEAQ
TIKLLNVCRMRDTPIITFINKLDRESRAPIELLDEIETTLGMECAPMTWPIGMGKTFRGV
YSLVNDSILFFDPRAEKGTSETIQGLDNPRLDELIGYQAQELRVDIELVRGASNEFNKER
YLSGKQTPVFFGSAVNNFGVQSLLDAVVDLSPPPQARNTATRAVAANEDKFAGFVFKIQA
NMDPKHRDRIAFLRVCSGRFERGMKIKQVATGKMLNVNNAITFMAQDRTIMDEAYSGDII
GIPNHGTIKLGDTFTENEALKFIGIPSFAPEFFRRARIKNPMKMKQLQIGLKQLSEEGAA
QLFRPLLSNDLILGAVGMLQFDVVAHRLEHEYGVDMVFEPYDCYTARWLKGSDADLKAIA
DKYGFNVGLDSADDYVYLAPNRVNLQMAQERYPDIQFMETREIA