Protein Info for GFF988 in Variovorax sp. SCN45

Annotation: LSU rRNA pseudouridine(1911/1915/1917) synthase (EC 5.4.99.23)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00005: pseudouridine synthase, RluA family
  • amino acids 34 to 323 (290 residues), 267.3 bits, E=8.4e-84
• PF01479: S4
  • amino acids 37 to 81 (45 residues), 29.1 bits, 6.6e-11
• PF00849: PseudoU_synth_2
  • amino acids 113 to 268 (156 residues), 99.7 bits, E=2e-32

Best Hits

KEGG orthology group: K06180, ribosomal large subunit pseudouridine synthase D [EC: 5.4.99.12] (inferred from 92% identity to vpe:Varpa_3710)

Predicted SEED Role

"Ribosomal large subunit pseudouridine synthase D (EC 4.2.1.70)" in subsystem Ribosome biogenesis bacterial (EC 4.2.1.70)

MetaCyc Pathways

• pseudouridine degradation (1/2 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Protein Sequence (332 amino acids)

>GFF988 LSU rRNA pseudouridine(1911/1915/1917) synthase (EC 5.4.99.23) (Variovorax sp. SCN45)
LGNTPEHLEAAEHEEGADPAEASELRPFIIDSAQHGQRLDRALAALVPEFSRSYLQQLIE
AGAVELQGRTVTKVSAGVKAGQAGRIELRPTPQSQAFRPETMDLVTVYEDEHLRIVDKPA
GLVVHPAPGHWSGTLLNGLLALDPKAALLPRAGIVHRLDRDTSGLMVVARTRATMDAMVA
LIAAREVKRQYLAIGHKPWVGAAARQVDAPIGRDPRNRLRMAVVDLERHAGKTARTLIER
LDSHSEGCVVRCTLETGRTHQIRVHMASIGHPLVGDALYGGAPAVGLSRQALHAFRLAFV
HPVTQEPMEFRSPPPPDLIQALQSWGLVYNRA